A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting

Molecular Therapy - Methods & Clinical Development - Tập 20 - Trang 587-600 - 2021
Elisa Murenu1,2, Marina Pavlou1,2, Lisa Richter3, Kleopatra Rapti4,5, Sabrina Just6,7, Jasmina Cehajic-Kapetanovic8, Neda Tafrishi9, Andrew Hayes10, Rachel Scholey10, Robert Lucas10, Hildegard Büning6,7,11, Dirk Grimm4,5,12, Stylianos Michalakis1,2
1Department of Ophthalmology, Ludwig-Maximilians-University Munich, 80336 Munich, Germany
2Department of Pharmacy, Ludwig-Maximilians-University Munich, 81377 Munich, Germany
3Core Facility Flow Cytometry, Biomedical Center, Ludwig-Maximilians-University Munich, 82152 Planegg-Martinsried, Germany
4Department of Infectious Diseases/Virology, Medical Faculty, University of Heidelberg, 69120 Heidelberg, Germany
5Bioquant Center, University of Heidelberg, 69120 Heidelberg, Germany
6Laboratory for Infection Biology and Gene Transfer, Institute of Experimental Hematology, Hannover Medical School, 30625 Hannover, Germany
7REBIRTH – Research Center for Translational Regenerative Medicine, Hannover Medical School, 30625 Hannover, Germany
8Nuffield Laboratory of Ophthalmology, Nuffield Department of Clinical Neurosciences, Oxford University and Oxford University Hospitals, Oxford OX3 9DU, UK
9Core Facility Flow Cytometry, Gene Center, BioSysM, Ludwig-Maximilians-University Munich, 81377 Munich, Germany
10Center for Biological Timing & School of Biological Sciences, Faculty of Biology Medicine and Health, University of Manchester, Manchester M13 9PL, UK
11German Center for Infection Research (DZIF), partner site, Hannover, Germany
12German Center for Infection Research (DZIF) and German Center for Cardiovascular Research (DZHK), partner site, Heidelberg, Germany

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