Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Một phương pháp sắc ký khí-mass spectrometry khối lượng kép hai bước để đo lường nhiều chất ô nhiễm môi trường trong huyết tương người
Tóm tắt
Cá nhân tiếp xúc với nhiều loại hóa chất trong suốt cuộc đời, tuy nhiên hiểu biết hiện tại về độc tính hỗn hợp vẫn còn hạn chế. Chúng tôi giới thiệu một phương pháp phân tích hai bước sử dụng sắc ký khí và khối phổ ba khối để yêu cầu ít hơn 1 mL mẫu. Phương pháp này được áp dụng cho 183 mẫu huyết tương từ một quần thể nghiên cứu bao gồm trẻ em mắc rối loạn phổ tự kỷ, cha mẹ của họ và trẻ em bình thường không có liên quan. Chúng tôi đã chọn 156 hợp chất hóa chất môi trường và loại trừ các hóa chất có tỷ lệ phát hiện dưới 20% trong đoàn nghiên cứu của chúng tôi (n = 61), cũng như các chất không phù hợp với các phương pháp chiết xuất và phân tích đã chọn (n = 34). Phương pháp mục tiêu sau đó tập trung vào các hóa chất còn lại (n = 61) cộng với 8 polychlorinated biphenyls (PCBs) bổ sung. Các chất ô nhiễm dai dẳng, chẳng hạn như p,p′-dichlorodiphenyldichloroethylene (p,p′-DDE) và các đồng phân PCB 118 và 180, đã được phát hiện với tần suất cao và một số hóa chất chưa được báo cáo trước đó, bao gồm 2,4,6-trichlorophenol, isosafrole, và hexachlorobutadiene, đã được phát hiện thường xuyên trong đoàn nghiên cứu của chúng tôi. Công trình này nhấn mạnh lợi ích của việc sử dụng phương pháp phân tích nhiều bước trong các nghiên cứu tiếp xúc và chứng minh tính hiệu quả của các phương pháp như vậy trong việc báo cáo thông tin mới về các tiếp xúc ô nhiễm chưa được nghiên cứu trước đó.
Từ khóa
#độc tính hỗn hợp #sắc ký khí #khối phổ #ô nhiễm môi trường #polychlorinated biphenylsTài liệu tham khảo
Agency for Toxic Substances and Disease Registry (1994) Toxicological profile for hexachlorobutadiene. U.S. Department of Health and Human Services, Public Health Service, Atlanta
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