A study of the products of the hydrolysis of the xylan ofMelilotus albus by endo-1 4-β-xylanase

Chemistry of Natural Compounds - Tập 16 - Trang 338-342 - 1980
M. S. Dudkin, N. A. Rodionova, I. S. Kazanskaya, I. V. Gorbacheva, E. I. Kozarez, N. A. Denisyuk

Tóm tắt

The enzymatic hydrolysis of a 4-0-methylglucuronoxylan of white sweetclover (Melilotus albus) by a highly purified homogeneous endo-1, 4-β-xylanase fromAspergillus niger 14 has shown that the enzyme hydrolyzes 97% of the polysaccharide in 72 h. Acidic and neutral oligosaccharides were found in the hydrolysate after the action of the enzyme. An investigation of the hexauronic acid isolated has shown that the glucuronic acid is attached to the nonreducing end of the xylooligosaccharide, which demonstrates the specific action of the enzyme on the polysaccharide.

Tài liệu tham khảo

N. A. Rodionova, R. V. Feniksova, and I. V. Itomlenskite, Khim. Drev.,8, 127 (1971). I. V. Gorbacheva and N. A. Rodionova, Biochem. Biophys. Acta,484, 79 (1977). N. A. Rodionova, I. V. Gorbacheva, and V. A. Buivid, Biokhimiya,42, No. 4, 659 (1977). R. F. H. Dekker and G. N. Richards, Adv. Carbohydr. Chem.,32, 346 (1976). T. E. Timell, Svensk. Papperstidn.,65, 435 (1962). M. Sinner, Mitt. Bundesforschunganstalt Forst, Holz-Wirtsch.,93, 257 (1973). M. S. Dudkin and N. G. Shkantova, Abstracts of Lectures at an All-Union Conference on Processing Products of the Timber and Agricultural Industries [in Russian], Riga (1973), p. 24. G. H. Roe, J. Biol. Chem.,212, 335 (1955). E. L. Hirst and E. Percival, in: Methods of Carbohydrate Chemistry (R. L. Whistler, ed.), Vol. 5, Academic Press, New York (1965), pp. 289–290. C. J. Giang and R. H. Marchessault, J. Polymer Sci.,39, 269 (1959).