A steroidal derivative from Trigonella foenum graecum L. that induces apoptosis in vitro and in vivo

Hutchinson, 2010, Breast cancer: challenges, controversies, breakthroughs, Nat Rev Clin Oncol, 10, 669, 10.1038/nrclinonc.2010.192 Kitai, 2017, Induction of G2/M arrest and apoptosis through mitochondria pathway by a dimer sesquiterpene lactone from Smallanthus sonchifolius in HeLa cells, J Food Drug Anal, 25, 619, 10.1016/j.jfda.2016.10.005 Patil, 2014, Holistic approach of Trigonella foenum-graecum in phytochemistry and pharmacology- A review, Curr Trends Technol Sci, 3, 34 Kandhare, 2015, Effect of glycosides based standardized fenugreek seed extract in bleomycin-induced pulmonary fibrosis in rats: decisive role of Bax, Nrf2, NF-jB, Muc5ac, TNF-a and IL-1b, Chem Biol Interact, 237, 151, 10.1016/j.cbi.2015.06.019 Bin-Hafeez, 2003, Immunomodulatory effects of fenugreek (Trigonella foenum graecum L.) extract in mice, Int Immunopharmacol, 3, 257, 10.1016/S1567-5769(02)00292-8 Khalil, 2015, Trigonella foenum (Fenugreek) induced apoptosis in hepatocellular carcinoma cell line, HepG2, mediated by upregulation of p53 and proliferating cell nuclear antigen, BioMed Res Int, 11 Prakash, 2014, Trigonella foenum-graecum (fenugreek) extract exhibit anti-cancer activity when checked against Human Cancer Cell Line MDAMD453. Journal of Environmental Science, Comput Sci Eng Technol, 3, 1196 Raju, 2004, Diosgenin, a steroid saponin of Trigonella foenum-graecum, inhibits azoxymethane induced aberrant crypt foci formation in F344 rats and induces apoptosis in HT-29 human colon cancer cells, Cancer Epidemiol Biomarkers Prev, 13, 1392, 10.1158/1055-9965.1392.13.8 Kaviarasan, 2007, In vitro studies on antiradical and antioxidant activities of fenugreek (Trigonella foenum graecum) seeds, Food Chem, 103, 31, 10.1016/j.foodchem.2006.05.064 Zargar, 2014, Protective effect of Trigonella foenum-graecum on thioacetamide induced hepatotoxicity in rats, Saudi J Biol Sci, 21, 139, 10.1016/j.sjbs.2013.09.002 Gaur, 2013, Neurobehavioral assessment of hydroalcoholic extract of Trigonella foenum-graecum seeds in rodent models of Parkinson's disease, Pharm Biol, 51, 550, 10.3109/13880209.2012.747547 Balaraman, 2006, Antihypertensive Effect of Trigonella foenum-greacum. seeds in experimentally induced hypertension in rats, Pharm Biol, 44, 568, 10.1080/13880200600896538 Steels, 2017, Efficacy of a proprietary Trigonella foenum-graecum L.de-husked seed extract in reducing menopausal symptoms in otherwise healthy women: a double-blind, randomized, placebo-controlled study, Phytother Res, 31, 1316, 10.1002/ptr.5856 Liu, 2012, Compounds in functional food fenugreek spice exhibit anti-inflammatory and antioxidant activities, Food Chem, 131, 1187, 10.1016/j.foodchem.2011.09.102 Basch, 2003, Therapeutic applications of fenugreek, Alternative Med Rev, 8, 20 Ouzir, 2016, Toxicological properties of fenugreek (Trigonella foenum graecum), Food Chem Toxicol, 96, 145, 10.1016/j.fct.2016.08.003 Zhou, 2012, Trigonelline: a plant alkaloid with therapeutic potential for diabetes and central nervous system disease, Curr Med Chem, 19, 3523, 10.2174/092986712801323171 He, 2014, Isolation and purification of six bioactive compounds from the seeds of Trigonella foenum-graecum L. using high-speed counter-current chromatography, Separ Sci Technol, 49, 580, 10.1080/01496395.2013.836229 Pang, 2011, Two new furostanol saponins from the seeds of Trigonella foenum-graecum, J Asian Nat Prod Res, 13, 611, 10.1080/10286020.2011.579073 Lambert, 2001, Potential interaction between warfarin and boldo-fenugreek, Pharmacotherapy, 21, 509, 10.1592/phco.21.5.509.34492 Yen, 2018, Antioxidant activity and anticancer effect of ethanolic and aqueous extracts of the roots of Ficus beecheyana and their phenolic components, J Food Drug Anal, 26, 182, 10.1016/j.jfda.2017.02.002 Collymore, 2014, Efficacy and safety of 5 anesthetics in adult zebrafish (Danio rerio), J Am Assoc Lab Anim Sci, 53, 198 Bai, 2018, A novel protein from edible fungi Cordyceps militaris that induces apoptosis, J Food Drug Anal, 26, 21, 10.1016/j.jfda.2016.10.013 Tran, 2011, Trypan blue dye enters viable cells incubated with the pore-forming toxin HlyII of Bacillus cereus, PLoS One, 6, e22876, 10.1371/journal.pone.0022876 Garzon, 2012, A combined approach for the assessment of cell viability and cell functionality of human fibrochondrocytes for use in tissue engineering, PLoS One, 7, e51961, 10.1371/journal.pone.0051961 Crowley, 2016, Analyzing cell death by nuclear staining with hoechst 33342, Cold Spring Harb Protoc, 9 Singh, 1995, Use of DNA- binding flourochromes for the nuclear staining in fungi, 53 Liu, 1997, DFF, a heterodimeric protein that functions downstream of caspase 3 to trigger DNA fragmentation during apoptosis, Cell, 89, 175, 10.1016/S0092-8674(00)80197-X Datta, 1997, Caspase- 3 mediated cleavage of protein kinase C theta in induction of apoptosis, J Biol Chem, 272, 20317, 10.1074/jbc.272.33.20317 Brábek, 2010, The role of the tissue microenvironment in the regulation of cancer cell motility and invasion, Cell Commun Signal, 8, 22, 10.1186/1478-811X-8-22