A simplified method for the culturing of primary adult rat and human hepatocytes as multicellular spheroids

Green, C. E.; Segall, H. J.; Byard, J. L. Metabolism, cytotoxicity, and genotoxicity of the pyrrolizidine alkaloid senecionine in primary cultures of rat hepatocytes. Toxicol. Appl. Pharmacol. 60:176–185; 1981.

Guigoz, Y.; Werffeli, P.; Favre, D., et al. Aggregate cultures of fetal rat liver cells: development and maintenance of liver gene expression. Biology of the Cell 60:163–171; 1987.

Kane, R. E.; Tector, J.; Brems, J. J., et al. Sulfation and glucuronidation of acetaminophen by cultured hepatocytes reproducingin vivo sex-differences in conjugation on Matrigel and Type 1 collagen. In Vitro Cell. Dev. Biol. 27A:953–960; 1991.

Koide, N.; Sakaguchi, K.; Koide, Y., et al. Formation of multicellular spheroids composed of adult rat hepatocytes in dishes with positively charged surfaces and under other nonadherent environment. Exp. Cell Res. 186:227–235; 1990.

Landry, J. D.; Bernier, C.; Ouellet, R., et al. Spherical aggregate culture of rat liver cells: histiotypic reorganization, biomatrix deposition, and maintenance of functional activities. J. Cell Biol. 101:914–923; 1985.

Li, A. P. Growth of mammalian cells as unattached cultures on nontissue culture plates. J. Tissue Culture Meth. 6:71–74; 1980.

Li, A. P.; Myers, C. A.; Roque, M. A., et al. Epidermal growth factor induction of DNA synthesis in primary cultures of human hepatocytes. In Vitro Cell. Dev. Biol. 27A:831–833; 1991.

Li, A. P.; Roque, M. A.; Beck, D. J., et al. Isolation and culturing of hepatocytes from human livers. J. Tissue Culture Meth. 14:139–146; 1992.

Li, A. P.; Myers, C. A.; Kaminski, D. L. Gene transfer in primary cultures in human hepatocytes. In Vitro Cell. Dev. Biol. In press; 1992a.

Loretz, L. J.; Wilson, A. G. E.; Li, A. P. Promutagen activation by freshly isolated and cryopreserved rat hepatocytes. Environ. Mol. Mutag. 12:335–341; 1988.

Loretz, L. J.; Li, A. P.; Flye, M. W., et al. Optimization of cryopreservation procedures for rat and human hepatocytes. Xenobiotica 19:489–498; 1989.

Reese, J. A.; Byard, J. L. Isolation and culture of adult hepatocytes from liver biopsies. In Vitro 17:935–940; 1981.

Sakaguchi, K.; Koide, N.; Asano, K., et al. Promotion of spheroid assembly of adult rat hepatocytes by some factor(s) present in the initial 6-hour conditioned medium of the primary culture. Pathobiol. 59:351–356; 1991.

Shinji, N.; Koide, N.; Tsuji, T. Glycosaminoglycans partially substitutes for proteoglycans in spheroid formation of adult rat hepatocytes in primary culture. Cell Struct. Funct. 13:179–188; 1988.

Tong, J. Z.; Bernard, O.; Alvarez, F. Long-term culture of rat liver cell spheroids in hormonally defined media. Exp. Cell Res. 189:87–92; 1990.

Tong, J. Z.; De Lagausie, P.; Furlan, V., et al. Long-term culture of adult rat hepatocyte spheroids. Exp. Cell Res. 200:326–332; 1992.

Yuhas, J. M.; Li, A. P. Growth fraction as the major determinant of multicellular tumor spheroid growth rate. Cancer Res. 38:1528–1532; 1978.

Yuhas, J. M.; Li, A. P.; Martinez, A. O., et al. A simplified method for production and gowth of multicellular tumor spheroids. Cancer Res. 37:3639–3643; 1977.