A simple stopped assay for fatty acid amide hydrolase avoiding the use of a chloroform extraction phase

Journal of Biochemical and Biophysical Methods - Tập 60 - Trang 171-177 - 2004
Linda Boldrup1, Sandy J Wilson2, Ann J Barbier2, Christopher J Fowler1
1Department of Pharmacology and Clinical Neuroscience, Umeå University, SE901 87 Umeå, Sweden
2Johnson & Johnson Pharmaceutical Research & Development LLC Neuroscience, 3210 Merryfield Row, San Diego, CA 92121, USA

Tài liệu tham khảo

Wilson, 2001, Endogenous cannabinoids mediate retrograde signalling at hippocampal synapses, Nature, 410, 588, 10.1038/35069076 Pertwee, 2001, Cannabinoid receptors and pain, Prog. Neurobiol., 63, 569, 10.1016/S0301-0082(00)00031-9 Fowler, 2003, Plant-derived, synthetic and endogenous cannabinoids as neuroprotective agents. Non-psychoactive cannabinoids, ‘entourage’ compounds and inhibitors of N-acylethanolamine breakdown as therapeutic strategies to avoid pyschotropic effects, Brain Res. Rev., 41, 26, 10.1016/S0165-0173(02)00218-7 Bifulco, 2002, Targeting the endocannabinoid system in cancer therapy: a call for further research, Nat. Med., 8, 547, 10.1038/nm0602-547 Deutsch, 1993, Enzymatic synthesis and degradation of anandamide, a cannabinoid receptor agonist, Biochem. Pharmacol., 46, 791, 10.1016/0006-2952(93)90486-G Cravatt, 2001, Supersensitivity to anandamide and enhanced endogenous cannabinoid signaling in mice lacking fatty acid amide hydrolase, Proc. Natl. Acad. Sci. U. S. A., 98, 9371, 10.1073/pnas.161191698 Fowler, 2001, Fatty acid amide hydrolase: biochemistry, pharmacology, and therapeutic possibilities for an enzyme hydrolyzing anandamide, 2-arachidonoylglycerol, palmitoylethanolamide, and oleamide, Biochem. Pharmacol., 62, 517, 10.1016/S0006-2952(01)00712-2 Kathuria, 2003, Modulation of anxiety through blockade of anandamide hydrolysis, Nat. Med., 9, 76, 10.1038/nm803 Schmid, 1985, Properties of rat liver N-acylethanolamine amidohydrolase, J. Biol. Chem., 260, 14145, 10.1016/S0021-9258(17)38695-7 Qin, 1998, Determination of anandamide amidase activity using ultraviolet-active amine derivatives and reverse-phase high-performance liquid chromatography, Anal. Biochem., 261, 8, 10.1006/abio.1998.2713 Maccarrone, 1999, A sensitive and specific radiochromatographic assay of fatty acid amide hydrolase, Anal. Biochem., 267, 314, 10.1006/abio.1998.2964 Thumser, 1997, A fluorescence displacement assay for the measurement of arachidonoyl ethanolamide (anandamide) and oleoyl amide (octadecenoamide) hydrolysis, Biochem. Pharmacol., 53, 433, 10.1016/S0006-2952(96)00720-4 Patricelli, 2001, Characterization and manipulation of the acyl chain selectivity of fatty acid amide hydrolase, Biochemistry, 40, 6107, 10.1021/bi002578r Bachur, 1966, Microsomal synthesis of fatty acid amides, J. Biol. Chem., 241, 1308, 10.1016/S0021-9258(18)96775-X Desarnaud, 1995, Anandamide amidohydrolase activity in rat brain microsomes. Identification and partial characterization, J. Biol. Chem., 270, 6030, 10.1074/jbc.270.11.6030 Omeir, 1995, Arachidonyl ethanolamide-[1,2-14C] as a substrate for anandamide amidase, Life Sci., 56, 1999, 10.1016/0024-3205(95)00181-5 Wilson, 2003, A high-throughput-compatible assay for determining the activity of fatty acid amide hydrolase, Anal. Biochem., 318, 270, 10.1016/S0003-2697(03)00217-3 Harrington, 1990, Lowry protein assay containing sodium dodecyl sulfate in microtiter plates from protein determination on fractions from brain tissue, Anal. Biochem., 186, 285, 10.1016/0003-2697(90)90081-J Folch, 1957, A simple method for the isolation and purification of total lipides from animal tissue, J. Biol. Chem., 226, 497, 10.1016/S0021-9258(18)64849-5 Bligh, 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37, 911, 10.1139/o59-099 Hillard, 1995, Characterization of the kinetics and distribution of N-arachidonoylethanolamine (anandamide) hydrolysis by rat brain, Biochim. Biophys. Acta, 1257, 249, 10.1016/0005-2760(95)00087-S Jonsson, 2001, Effects of homologues and analogues of palmitoylethanolamide upon the inactivation of the endocannabinoid anandamide, Br. J. Pharmacol., 133, 1263, 10.1038/sj.bjp.0704199 Fowler, 2003, Acidic nonsteroidal antiinflamatory drugs inhibit rat brain fatty acid amide hydrolase in a pH-dependent manner, J. Eng. Inhib. Med. Chem., 18, 55, 10.1080/1475636021000049726