A simple method to detect contaminating peptides and amino acids in large-scale ganglioside preparations

Wiegandt, 1985, Gangliosides, Vol. 10, 199 Yu, 1989, Structure and localization of gangliosides, 1 Sabel, 1986, Pharmacological treatment of central nervous system injury, Nature, 323, 493, 10.1038/323493a0 Triban, 1989, Ganglioside treatment and improved axonal regeneration capacity in experimental diabetic neuropathy, Diabetes, 38, 1012, 10.2337/diabetes.38.8.1012 Geisler, 1991, Motor recovery following human spinal cord injury. A randomized placebo controlled trial with GM1 ganglioside, N Engl J Med, 324, 1829, 10.1056/NEJM199106273242601 Bidlingmeyer, 1984, Rapid analysis of amino acids using pre-column derivatization, J Chromatogr, 336, 93, 10.1016/S0378-4347(00)85133-6 Lowry, 1951, Protein measurement with the Folin phenol reagent, J Biol Chem, 193, 265, 10.1016/S0021-9258(19)52451-6 Meltzer, 1987, Gas-phase hydrolysis of proteins and peptides, Anal Biochem, 160, 356, 10.1016/0003-2697(87)90060-1 Moore, 1958, Chromatography of amino acids on sulfonated polystyrene resins. An improved system, Anal Chem, 30, 1185, 10.1021/ac60139a005 Hamilton, 1963, Ion exchange chromatography of amino acids. A single column, high resolving, fully automatic procedure, Anal Chem, 35, 2055, 10.1021/ac60206a024 1989, J Biol Stand, 17, 203, 10.1016/0092-1157(89)90012-7 Peterson, 1979, Review of Folin phenol protein quantitation of Lowry, Rosebrough, Farr and Randall, Anal Biochem, 100, 201, 10.1016/0003-2697(79)90222-7 Chou, 1960, Chromogenic groupings in the Lowry protein determinations, Biochem J, 75, 109, 10.1042/bj0750109 Eichberg, 1969, Interference by oxidized lipids in the determination of protein by the Lowry procedure, Anal Biochem, 30, 386, 10.1016/0003-2697(69)90131-6 Nishio, 1982, Protein assays in very diluted solutions, Anal Biochem, 126, 239, 10.1016/0003-2697(82)90509-7