A rapid staining method showing AgNOR and DNA in pathological diagnosis

Chinese Journal of Cancer Research - Tập 6 - Trang 139-143 - 1994
Zhijin Gong1, Weiqiang Zheng1, Rongzhou Zhan1, Wenzhao Tao1
1Department of Pathology, Second Military Medical University, Shanghai

Tóm tắt

In order to satisfy the need of rapid clinical diagnosis, a series of experimental studies for AgNOR (Argyrophilic NOR-associated proteins) and DNA (Deosyribonucleic acid) staining was compared, from which a stable staining solution was selected to show mucin residence. Using the referred tables, with reference to the corresponding relation of the staining solution with temperature and time, reliable staining results for AgNOR and DNA could be obtained. These two modified staining methods are simple, reliable and easy to operate, able to provide good contrast for pathological diagnosis.

Tài liệu tham khảo

Crocker J, Nar P. Nucleolar organizer regions in lymphomas. J Pathol 1987; 151:111. Smith R, Grocker J. Evaluation of nucleolar organizer region- associated proteins in breast malignancy. Histopathol 1988; 12:113 1989; 190.