A rapid method for the purification of antibody–enzyme conjugates

Canadian Science Publishing - Tập 57 Số 3 - Trang 286-288 - 1979
Martin A. Page, Marilyne Audette, Michel Caron

Tóm tắt

A method is described for a rapid and efficient separation of enzyme-labelled antibodies from the free enzyme following the coupling reaction. A single passage of the reaction mixture on a protein A – Sepharose CL-4B column gave a sharp separation of the free enzyme from the conjugate.

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