A rapid biosynthetic method for the preparation of radioactive phosphatidyl-CMP(CDP-diacylglycerol) of high specific activity.

Agranoff, 1958, The enzymatic synthesis of inositol phosphatide, J. Biol. Chem., 233, 1077, 10.1016/S0021-9258(19)77342-6 Paulus, 1960, The enzymatic synthesis of inositol monophosphatide, J. Biol. Chem., 235, 1303, 10.1016/S0021-9258(18)69403-7 Thompson, 1963, Biosynthesis of phosphatidylinositol in rat brain, Biochem. J., 87, 136, 10.1042/bj0870136 Kiyasu, 1963, The biosynthesis of phosphatidylglycerol, J. Biol. Chem., 238, 2293, 10.1016/S0021-9258(19)67968-8 Stanacev, 1973, Biochemistry of polyglycerophosphatides. The mechanism of cardiolipin biosynthesis in isolated mitochondria, Can. J. Biochem., 51, 286, 10.1139/o73-035 Hirschberg, 1972, Mechanism of the enzymatic synthesis of cardiolipin in Escherichia coli, Proc. Nat. Acad. Sci. USA, 69, 648, 10.1073/pnas.69.3.648 Hostetler, 1972, The mechanism of cardiolipin biosynthesis in liver mitochondria, Biochim. Biophys. Acta., 260, 507, 10.1016/0005-2760(72)90065-3 Kanfer, 1964, Metabolism and function of bacterial lipids II. Biosynthesis of phospholipids in Escherichia coli, J. Biol. Chem., 239, 1720, 10.1016/S0021-9258(18)91247-0 Thompson, 1975, Isolation and characterization of cytidine diphosphate-diglyceride from beef liver, J. Biol. Chem., 250, 6779, 10.1016/S0021-9258(19)40999-X Thompson, 1976, Cytidine diphosphate-diglyceride of bovine brain, Eur. J. Biochem., 65, 107, 10.1111/j.1432-1033.1976.tb10394.x Agranoff, 1963, Cytidine diphosphate-dl-dipalmitin, Biochem. Prep., 10, 47 Prottey, 1967, The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas, Biochem. J., 105, 379, 10.1042/bj1050379 Eichberg, 1973, Stimulation of 32P1 incorporation into phosphatidylinositol and phosphatidylglycerol by catecholamines and β-adrenergic receptor blocking agents in rat pineal organ cultures, J. Biol. Chem., 248, 3615, 10.1016/S0021-9258(19)43973-2 Hauser, 1975, Identification of cytidine diphosphate-diglyceride in the pineal gland of the rat and its accumulation in the presence of dl-propranolol, J. Biol. Chem., 250, 105, 10.1016/S0021-9258(19)41987-X Raetz, 1972, A membrane-bound pyrophosphatase in Escherichia coli catalyzing the hydrolysis of cytidine diphosphate-diglyceride, J. Biol. Chem., 247, 2245, 10.1016/S0021-9258(19)45521-X Folch, 1957, A simple method for the isolation and purification of total lipids from animal tissues, J. Biol. Chem., 226, 497, 10.1016/S0021-9258(18)64849-5 Petzold, 1967, The biosynthesis of cytidine diphosphate-diglyceride by embryonic chick brain, J. Biol. Chem., 242, 1187, 10.1016/S0021-9258(18)96162-4 Bishop, 1976, Studies on the formation by rat brain preparations of CDP-diglyceride from CTP and phosphatidic acids of varying fatty acid compositions, Can. J. Biochem., 54, 249, 10.1139/o76-038 Hajra, 1974, On extraction of acyl and alkyl dihydroxyacetone phosphate from incubation mixtures, Lipids., 9, 502, 10.1007/BF02532495 Bjerve, 1974, The selective loss of lysophospholipids in some commonly used lipid-extraction procedures, Anal. Biochem., 58, 238, 10.1016/0003-2697(74)90463-1 Freinkel, 1975, Inter-relations between the phospholipids of rat pancreatic islets during glucose stimulation and their response to medium inositol and tetracaine, Eur. J. Biochem., 59, 245, 10.1111/j.1432-1033.1975.tb02448.x Abdel-Latif, 1976, Effects of dl-propranolol on the synthesis of glycerolipids by rabbit iris muscle, Biochem. Pharmacol., 25, 1697, 10.1016/0006-2952(76)90401-9 Bazán, 1976, De novo glycerolipid biosynthesis in the toad and cattle retina. Redirecting of the pathway by propranolol and phentolamine, Adv. Exp. Med. Biol., 72, 139, 10.1007/978-1-4684-0955-0_13 Eichberg, 1978, Modification of phosphatidylinositol metabolism by propranolol and local anesthetics Margraff, 1976, A biosynthetic method for the preparation of high specific activity. 32P-labeled phospholipids. J, Lipid Res., 17, 622, 10.1016/S0022-2275(20)41734-1