A ramie (Boehmeria nivea) bZIP transcription factor BnbZIP3 positively regulates drought, salinity and heavy metal tolerance
Tóm tắt
bZIP genes play important roles in the regulation of many developmental and physiological processes and adaptive responses to the environment in plants. In the present study, a novel bZIP gene, designated as BnbZIP3 (Genbank accession number: KP462877), was cloned from ramie plants based on the sequence of Unigene6582 fragment in ramie transcriptome using rapid amplification of cDNA ends (RACE) technique and real-time PCR. Results indicated that BnbZIP3 had a 999-bp open reading frame, encoding a 332 amino acid protein that containing a characteristic bZIP domain, and shared high sequence identities with bZIP factors from other plants. Translational fusion of BnbZIP3 with EGFP showed subcellular localization of the protein in nucleus. Transcripts of BnbZIP3 were found in different tissues of ramie plants. Expression of BnbZIP3 was induced by drought, high salinity and ABA treatments. The promoter of BnbZIP3 contained many cis-acting elements involved in multiple stress responses. Overexpression of BnbZIP3 in transgenic Arabidopsis plants inhibited the growth of roots under normal growth conditions, whereas it improved the growth of roots under dehydration, salinity and heavy metal Cd stress, and increased germination of seeds under drought conditions. Thus, BnbZIP3 may positively regulate stress tolerance and be used to engineer enhanced drought, salt and heavy metal tolerance of ramie varieties or other industrial crops.
Tài liệu tham khảo
Abe HT, Urao T, Ito M, Seki K, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidop AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators abscisic acid signaling. Plant Cell 15:63–78
Ataei S (2014) Promoter regulation of the Craterostigma plantagineum Cp LEA-like 11-24 gene and functional characterization of its transcription factor (CpbZIP1). PhD thesis, Rheinischen Friedrich-Wilhelms-Universität, Bonn
Cambra K, Martinez T, Urzelai A, Alonso E (1999) Risk analysis of area near a lead-and cadmium-contaminated industrial site. J Environ Health 8:527–540
Chen H, Chen W, Zhou JL, He H, Chen LB, Chen HD, Deng XW (2012) Basic leucine zipper transcription factor OsbZIP16 positively regulates drought resistance in rice. Plant Sci 193–194:8–17
Choi H, Hong J, Ha J, Kang J, Kim SY (2000) ABFs, a family of ABA-responsive element binding factors. J Biol Chem 275:1723–1730
Clough SJ, Bent AF (1998) Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. Plant J 16:735–743
Corrêa LGG, Riaño-Pachón DM, Schrago CG, Vicentini dos Santos R, Mueller-Roeber B, Vincentz M (2008) The role of bZIP transcription factors in green plant evolution: adaptive features emerging from four founder genes. PLoS ONE 3:e2944
Fujita Y, Fujita M, Satoh R, Maruyama K, Parvez MM, Seki M, Hiratsu K, Ohme-Takagi M, Shinozaki K, Yamaguchi-Shinozaki K (2005) AREB1 is a transcription activator of novel ABRE-dependent ABA signaling that enhances drought stress tolerance in Arabidopsis. Plant Cell 17:3470–3488
Fukazawa J et al (2000) Repression of shoot growth, a bZIP transcriptional activator, regulates cell elongation by controlling the level of gibberellins. Plant Cell 12:901–915
Fukumoto T, Kano A, Ohtani K, Inoue M, Yoshihara A, Izumori K, Tajima S, Shigematsu Y, Tanaka K, Ohkouchi T, Tada Y, Ichimura K, Gomi K, Akimitsu K (2013) Phosphorylation of D-allose by hexokinase involved in regulation of OsABF1 expression for growth inhibition in Oryza sativa L. Planta 237:1379–1391
Givens CR, Zhang PL, Bair SR, Mellon SH (1994) Transcriptional regulation of rat cytochrome P450c17 expression in mouse leydig MA-10 and adrenal Y-1 Cells: identification of a single protein that mediates both basal and cAMP-induced activities. DNA Cell Biol 13:1087–1098
Gupta UC, Gupta SC (1998) Trace element toxicity relationships to crop production and livestock and human health: implications for management. Commun Soil Sci Plant Anal 29:1491–1522
Huang XS, Liu JH, Chen XJ (2010) Overexpression of PtrABF gene, a bZIP transcription factor isolated from Ponciru strifoliata, enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes. BMC
Huang CJ, Zhao SY, Wang LC, Anjum SA, Chen M, Zhou HF, Zou CM (2013) Alteration in chlorophyll fluorescence, lipid peroxidation and antioxidant enzymes activities in hybrid ramie (Boehmeria nivea L.) under drought stress. AJCS 7:594–599
Huang CJ, Wei G, Jie YC, Wang LC, Zhou HF, Ran CY, Huang ZC, Jia HJ, Anjum SA (2014) Effects of concentrations of sodium chloride on photosynthesis, antioxidative enzymes, growth and fiber yield of hybrid ramie. Plant Physiol Biochem 76:86–93
Iven T, Strathmann A, Böttner S, Zwafink T, Heinekamp T, Guivarch A, Roitsch T, Dröge-Laser W (2010) Homo- and heterodimers of tobacco bZIP proteins counteract as positive or negative regulators of transcription during pollen development. Plant J 63:155–166
Jakoby M et al (2002) bZIP transcription factors in Arabidopsis. Trends in Plant Sci 7:106–111
Jin ZW, Xu W, Liu AZ (2014) Genomic surveys and expression analysis of bZIP gene family in castor bean (Ricinus communis L.). Planta 239:299–312
Kang JY, Choi HI, Im MY, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357
Kim JB, Kang JY, Kim SY (2004) Over-expression of a transcription factor regulating ABA-responsive gene expression confers multiple stress tolerance. Plant Biotechnol J 2:459–466
Lee SC, Choi HW, Hwang IS, Choi DS, Hwang BK (2006) Functional roles of the pepper pathogen-induced bZIP transcription factor, CAbZIP1, in enhanced resistance to pathogen infection and environmental stresses. Planta 224:1209–1225
Lee SJ, Kang JY, Park HJ, Kim MD, Bae MS, Choi HI, Kim SY (2010) DREB2C interacts with ABF2, a bZIP protein regulating abscisic acid-responsive gene expression, and its overexpression affects abscisic acid sensitivity. Plant Physiol 153:716–727
Li J, Park E, Von Arnim A, Nebenführ A (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. Plant Methods 20:5–6
Liao Y, Zou HF, Wei W, Hao YJ, Tian AG, Huang J, Liu YF, Zhang JS, Chen SY (2008) Soybean GmbZIP44, GmbZIP62 and GmbZIP78 genes function as negative regulator of ABA signaling and confer salt and freezing tolerance in transgenic Arabidopsis. Planta 228:225–240
Liu CT, Wu YB, Wang XP (2012) bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice. Planta 235:1157–1169
Liu X, Wang Z, Wang L, Wu R, Phillips J, Deng X (2009) LEA 4 group genes from the resurrection plant Boea hygrometrica confer dehydration tolerance in transgenic tobacco. Plant Sci 176:90–98
Muñiz García MN, Giammaria V, Grandellis C, Téllez-In′ón Ulloa RM, Capiati DA (2012) Characterization of StABF1, a stress-responsive bZIP transcription factor from Solanum tuberosum L. that is phosphorylated by StCDPK2 in vitro. Planta 235:761–778
Nijhawan A, Jain M, Tyagi AK, Khurana JP (2008) Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol 146:333–350
Pontier D, Miao ZH, Lam E (2001) Trans-dominant suppression of plant TGA factors reveals their negative and positive roles in plant defense responses. Plant J 27:529–538
Pourabed E, Ghane Golmohamadi F, Soleymani Monfared P, Razavi SM, Shobbar ZS (2014) Basic Leucine Zipper family in barley: genome-wide characterization of members and expression analysis. Mol Biotechnol 57(1):12–26
Qi XZ, Guo LJ, Yang LY, Huang JS (2013) Foatf1, a bZIP transcription factor of Fusarium oxysporum f. sp. cubense, is involved in pathogenesis by regulating the oxidative stress responses of Cavendish banana (Musa spp.). Physiol Mol Plant Pathol 84:76–85
Rouster J, Leah R, Mundy J, Cameron-Mills V (1997) Identification of a methyl jasmonate-responsive region in the promoter of a lipoxygenase 1 gene expressed in barley grain. Plant J 11:513–523
Sakuma S, Pourkheirandish M, Matsumoto T, Koba T, Komatsuda T (2010) Duplication of a well-conserved homeodomain-leucine zipper transcription factor gene in barley generates a copy with more specific functions. Funct Integr Genomics 10:123–133
Silveira AB, Gauer L, Tomaz JP, Cardoso PR, Carmello-Guerreiro S, Vincentz M (2007) The Arabidopsis AtbZIP9 protein fused to the VP16 transcriptional activation domain alters leaf and vascular development. Plant Sci 172:1148–1156
Sohn KH, Lee SC, Jung HW, Hong JK, Hwang BK (2006) Expression and functional roles of the pepper pathogen-induced transcription factor RAV1 in bacterial disease resistance, and drought and salt stress tolerance. Plant Mol Biol 61:897–915
Tak H, Mhatre M (2013) Cloning and molecular characterization of a putative bZIP transcription factor VvbZIP23 from Vitis vinifera. Protoplasma 250:333–334
Thanh T, Chi VTQ, Abdullah MP, Omar H, Napis S (2012) Efficiency of ligation-mediated PCR and TAIL-PCR methods for isolation of RbcS promoter sequences from green microalga Ankistrodesmus convolutes. Mol Biol 46:58–64
Uno Y, Furihata T, Abe H, Yoshida R, Shinozaki K, Yamaguchi-Shinozaki K (2000) Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions. Proc Natl Acad Sci USA 97:11632–11637
Voigt O, Herzog B, Jakobshagen A, Pöggeler S (2013) bZIP transcription factor SmJLB1 regulates autophagy-related genes Smatg8 and Smatg4 and is required for fruiting-body development and vegetative growth in Sordaria macrospore. Fungal Genet Biol 61:50–60
Wang B, Zheng J, Liu YJ, Wang JH, Wang GY (2012) Cloning and characterization of the stress-induced bZIP gene ZmbZIP60 from maize. Mol Biol Rep 39:6319–6327
Yang K, Popova VO, Süthoff U, Lüking I, Dietz KJ, Golldack D (2009) The Arabidopsis basic leucine zipper transcription factor AtbZIP24 regulates complex transcriptional networks involved in abiotic stress resistance. Gene 436:45–55
Yang B, Zhou M, Shu WS, Lan CY, Ye ZH, Qiu RL, Jie YC, Cui GX, Wong MH (2010) Constitutional tolerance to heavy metals of a fiber crop, ramie (Boehmeria nivea), and its potential usage. Environ Pollut 158:551–558
Yin WB, Reinke AW, Szilágyi M, Emri T, Chiang YM, Keating AE, Pócsi I, Wang CCC, Keller NP (2013) bZIP transcription factors affecting secondary metabolism, sexual development and stress responses in Aspergillus nidulans. Microbiology 159:77–88
Yoshida T, Fujita Y, Sayama H, Kidokoro S, Maruyama K, Mizoi J, Shinozaki K, Shinozaki KY (2010) AREB1, AREB2, and ABF3 are master transcription factors that cooperatively regulate ABRE-dependent ABA signaling involved in drought stress tolerance and require ABA for full activation. Plant J 61:672–685
Zhang X, Wollenweber B, Jiang D, Liu F, Zhao J (2008) Water deficits and heat shock effects on photosynthesis of a transgenic Arabidopsis thaliana constitutively expressing ABP9, a bZIP transcription factor. J Exp Bot 59:839–848
Zhang Y, Zhang G, Xia N, Wang XJ, Huang LL, Kang ZS (2009) Cloning and characterization of a bZIP transcription factor gene in wheat and its expression in response to stripe rust pathogen infection and abiotic stresses. Physiol Mol Plant Pathol 73:88–94
Zhou JH, Yang QW, Lan CY, Ye ZH (2010) Heavy metal uptake and extraction potential of two Bechmeria nivea (L.) Gaud. (ramie) varieties associated with chemical reagents. Water Air Soil Pollut 211:359–366
Zou MJ, Guan YC, Ren HB, Zhang F, Chen F (2007) Characterization of alternative splicing products of bZIP transcription factors OsABI5. Biochem Biophys Res Commun 360:307–313