A quantitative approach to the analysis of supermicron dispersions by field-flow fractionation with UV-vis detectors. The application of an absolute method
Tóm tắt
Quantitative analysis in field-flow fractionation is becoming a necessary requirement for routine applications, instrumental optimization and scale-up to preparative separations. The use of detection systems which show complex dependence on sample characteristics (i.e. UV spectrometry) has hindered the application of quantitative methods of analysis in field-flow fractionation. A standardless model, shown valid in flow-through, homogeneous systems, is applied here to a heterogeneous system (dispersed supermicron particles) in field-flow fractionation by single peak area measurements. Absolute analysis in the fractionation of spherical silica particles for high-performance liquid chromatography column packing by gravitational field-flow fractionation with UV-Vis detectors is presented. It has been shown that for such samples extinction coefficients are independent of sample concentration and are determined by the size and density of the particles. The accuracy of such an approach to absolute analysis is discussed.
Tài liệu tham khảo
H. G. Barth, R. B. Flippen, Anal. Chem.67, 257R (1995).
J. C. Giddings, Science260, 1445 (1993).
H. Thielking, D. Roessner, W.-M. Kulicke, Anal. Chem.67, 3229 (1995).
F.-S. Yang, K. D. Caldwell, J. C. Giddings, J. Colloid Interface Sci.92, 81 (1983).
M. Blanda, P. Reschiglian, F. Dondi, Polym. Int.33, 61 (1994).
H. C. van de Hulst, in: “Light Scattering by Small Particles”, Dover Publications Inc., New York, NY, 1981.
G. Torsi, G. Chiavari, C. Laghi, A. M. Asmundsdottir, F. Fagioli, R. Vecchietti, J. Chromatogr.482, 207 (1989).
J. C. Giddings, M. N. Myers, K. D. Caldwell, J. W. Pav, J. Chromatogr.185, 261 (1979).
J. Pazourek, J. Chmelík, Chromatographia35, 591 (1993).
J. Pazourek, E. Urbánkova, J. Chmelík, J. Chromatogr.660, 113 (1994).
P. Reschiglian, G. Torsi, Chromatographia40, 467 (1995).
J. Pazourek, J. Chemelík, J. Chromatogr.715, 259 (1995).
P. Reschiglian, D. Melucci, G. Torsi, J. Chromatogr. A740, 245 (1996).
Ph. J. P. Cardot, J. Gerota, M. Martin, J. Chromatogr.568, 93 (1991).
A. Merino-Dugay, Ph. J. P. Cardot, M. Guernet, J. P. Andreux, J. Chromatogr.568, 73 (1992).
C. Bories, Ph. J. P. Cardot, V. Abramowski, C. Pons, A. Merino, C. Baron, J. Chromatogr.579, 141 (1992).
Ph. J. P. Cardot, C. Elgéa, M. Guernet, D. Godet, J. P. Andreux, J. Chromatogr. B654, 193 (1994).
J. Plocek, P. Konecny, J. Chmelík, J. Chromatogr. B656, 427 (1994).
A. Bernard, C. Bories, P. M. Loiseau, Ph. J. P. Cardot, J. Chromatogr. B664, 444 (1995).
F. P. Hochgeshong, in “Treatise on Analytical Chemistry”, I. M. Kolthoff & P. J. Elwing, eds., Vol. 5, Part I, Interscience, New York, NY, 1964, p. 3289.
D. H. Melik, H. S. Fogler, J. Colloid Int. Sci.92, 161 (1983).
D. A. Hangii, P. W. Carr, J. Liquid Chromatogr.7, 2323 (1984).
T. Kurti, J. F. MacGregor, in “Particle Size Distribution II, Assessment and Characterization”, ACS Symposium 472, T. Provder, ed., American Chemical Society, Washington D.C., 1991, Ch. 3.
R. W. Burke, E. R. Deardoff, O. Menis, J. Res. Natl. Bur. Stand.76A, 469 (1972).
G. Torsi, G. Chiavari, C. Laghi, A. M. Asmundsdottir, J. Chromatogr.518, 135 (1990).
S. K. Ratanathanawongs, J. C. Giddings, Chromatographia38, 545 (1994).
P. Reschiglian, D. Melucci, G. Torsi, private communication, FFF '95—Fifth International Symposium on Field-Flow Fractionation-Park City, UT, July 10–12, 1995.