A protocol for rheological characterization of hydrogels for tissue engineering strategies

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 102 Số 5 - Trang 1063-1073 - 2014
Jonathan M. Zuidema1, Christopher J Rivet1, Ryan J. Gilbert1, Faith A. Morrison2
1Department of Biomedical Engineering Center for Biotechnology and Interdisciplinary Studies Rensselaer Polytechnic Institute Troy New York 12180‐3590
2Department of Chemical Engineering Michigan Technological University Houghton Michigan 49931

Tóm tắt

AbstractHydrogels are studied extensively for many tissue engineering applications, and their mechanical properties influence both cellular and tissue compatibility. However, it is difficult to compare the mechanical properties of hydrogels between studies due to a lack of continuity between rheological protocols. This study outlines a straightforward protocol to accurately determine hydrogel equilibrium modulus and gelation time using a series of rheological tests. These protocols are applied to several hydrogel systems used within tissue engineering applications: agarose, collagen, fibrin, Matrigel™, and methylcellulose. The protocol is outlined in four steps: (1) Time sweep to determine the gelation time of the hydrogel. (2) Strain sweep to determine the linear‐viscoelastic region of the hydrogel with respect to strain. (3) Frequency sweep to determine the linear equilibrium modulus plateau of the hydrogel. (4) Time sweep with values obtained from strain and frequency sweeps to accurately report the equilibrium moduli and gelation time. Finally, the rheological characterization protocol was evaluated using a composite Matrigel™‐methylcellulose hydrogel blend whose mechanical properties were previously unknown. The protocol described herein provides a standardized approach for proper analysis of hydrogel rheological properties. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 1063–1073, 2014.

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Tài liệu tham khảo

10.1021/bm200083n

10.1016/j.actbio.2010.11.039

10.1016/j.biomaterials.2011.07.005

10.1126/science.1116995

10.1242/jcs.041186

10.1016/S0169-409X(01)00240-X

10.1021/ja907097t

10.1016/j.addr.2008.08.002

10.1016/j.biomaterials.2009.05.035

10.1016/j.addr.2010.02.001

10.1039/b919449p

10.3233/BIR-1993-303-407

10.1100/tsw.2003.15

Morrison FA, 2001, Understanding Rheology

Ferry JD, 1980, Viscoelastic Properties of Polymers

10.1021/bm020067

10.1016/j.actbio.2007.09.001

Bird RB, 1987, Dynamics of Polymeric Liquids

10.1016/j.ijpharm.2011.05.054

10.1122/1.549853

10.1021/ma00162a007

10.1122/1.549955

10.1016/j.jconrel.2004.05.003

10.1089/ten.tec.2009.0761

10.1529/biophysj.105.073114

10.1016/j.biomaterials.2010.06.046

10.1016/j.biomaterials.2008.02.005

10.1016/S0142-9612(00)00350-1

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Thông tin xuất bản

Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 102 Số 5

1063-1073

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