A physiological, rather than a superovulated, post‐implantation environment can attenuate the compromising effect of assisted reproductive techniques on gene expression in developing mice embryos

Molecular Reproduction and Development - Tập 82 Số 3 - Trang 191-206 - 2015
Elham Bonakdar1, M. A. Edriss1, Azizollah Bakhtari1, Farnoosh Jafarpour2, V. Asgari2, Sayyed Morteza Hosseini2, N. Sadeghi Boroujeni2, Mehdi Hajian2, Hamidreza Rahmani1, Mohammad Hossein Nasr‐Esfahani2
1Department of Animal Sciences, College of Agriculture, Isfahan University of Technology, Isfahan, Iran
2Department of Reproductive Biotechnology at Reproductive Biomedicine Research Center, Royan Institute for Biotechnology, ACECR, Isfahan, Iran

Tóm tắt

SUMMARYAssisted reproductive techniques (ARTs) may perturb the pre‐/peri‐conception microenvironments, which subsequently threaten the health of offspring. This study aimed to investigate the effects of superovulation, vitrification, in vitro culture, and embryo transfer on the expression of epigenetic modulators, imprinted genes, and pluripotency markers in expanded blastocysts and Day‐9.5 (D9.5) concepti. Results revealed that 53.4% (8/15) and 86.7% (13/15) of genes in the fetus and placenta, respectively, have similar patterns of transcription in all D9.5 concepti, despite the perturbed mRNA expression observed at the blastocyst stage for each embryo‐production technique. These observations indicate a counterbalancing of the abnormal expression pattern analyzed at the blastocyst stage during post‐implantation development, particularly when the uterus of a naturally synchronized foster mother is employed. Superovulation resulted in the most abnormal expression patterns compared to other treatment groups, although these same blastocysts were able to develop in a synchronized uterus. Thus, superovulation creates a hormonal environment that negatively affected gene expression and impairs fetal growth more adversely during post‐implantation development than other ART protocols, such as in vitro culture, vitrification, or embryo transfer—although each did contribute negatively to the implantation and development process. Together, these results may have implications for treating infertility in humans. Mol. Reprod. Dev. 82: 191–206, 2015. © 2015 Wiley Periodicals, Inc.

Từ khóa


Tài liệu tham khảo

10.1016/j.fertnstert.2011.02.029

10.1136/mp.50.1.34

10.1128/MCB.01755-07

10.1038/nature05515

10.1038/nrc3130

10.1038/nsmb.1821

10.1073/pnas.1119112109

10.1038/nsmb.2489

10.1038/nature00819

10.1086/341096

10.1016/S0960-9822(01)00311-6

10.1086/346031

10.1530/REP-12-0237

10.1242/dev.127.16.3419

10.1093/humrep/des255

10.1093/hmg/ddn055

10.1086/374824

10.1038/gim.2013.97

10.1002/j.1460-2075.1992.tb05507.x

10.1128/MCB.00993-07

10.4161/cc.9.10.11599

10.1016/j.stem.2014.08.003

10.1126/science.1160810

10.1016/j.tig.2012.01.005

10.1016/S0925-4773(02)00181-8

10.1086/423902

10.1242/dev.129.8.1983

10.1101/gad.1667008

10.1016/j.mrfmmm.2008.08.008

10.1016/j.fertnstert.2012.03.028

10.1007/s10815-013-0117-8

10.1038/cr.2011.189

10.1073/pnas.1014033108

10.1097/AOG.0b013e31822be65f

10.1093/hmg/ddp319

10.1016/j.ydbio.2007.10.033

10.1073/pnas.0607313103

10.1038/nrg887

Li E, 2002, Targeted mutation of the DNA methyltransferase gene results in embryonic lethality, Cell, 69, 915, 10.1016/0092-8674(92)90611-F

10.1016/j.devcel.2008.08.014

10.1093/molehr/gaq028

10.1186/1750-2187-3-15

10.1095/biolreprod.113.110411

10.1093/humupd/dms061

10.1095/biolreprod.111.096602

10.1093/hmg/ddp465

10.1023/A:1009227924235

10.1126/science.1216154

10.1093/hmg/ddi114

10.1038/ncb1519

10.1016/j.anireprosci.2005.06.027

10.1016/0092-8674(90)90597-8

10.1095/biolreprod.107.063453

10.1038/nature08829

10.1086/346030

10.1126/science.1063443

10.1006/dbio.1996.0172

Richa R, 2014, Hydroxymethylation of DNA: An epigenetic marker, EXCLI J, 13, 592

10.1002/j.1460-2075.1987.tb02607.x

10.1101/gad.12.14.2108

10.1242/dev.050849

10.1530/rep.1.00221

10.1006/dbio.2001.0501

10.1056/NEJMoa010806

10.1016/j.ceb.2013.02.013

10.1002/mrd.90016

10.1038/nature04914

10.1038/ng.864

10.1016/0092-8674(93)90160-R

10.1016/j.gene.2007.04.027

10.1093/humrep/deq278

10.1016/j.ydbio.2006.06.051

10.1093/humupd/dmn053

10.1016/j.stem.2013.01.016

10.1038/ncomms1240

10.1242/dev.128.10.1881

10.1093/humrep/der201

10.1074/jbc.M107435200

10.1101/gad.9.21.2635

10.1093/hmg/6.3.387

10.1016/j.tig.2008.08.004

Thông tin
Thông tin xuất bản

Molecular Reproduction and Development

Tập 82 Số 3

191-206

Thông tin tác giả