Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Một cuộc kiểm kê phát sinh loài về sự đa dạng toàn cầu của nấm kị khí trong ruột và một khung phân loại mới
Tóm tắt
Nấm kị khí bắt buộc thuộc ngành Neocallimastigomycota đóng vai trò quan trọng trong việc tiêu hóa thức ăn xơ tại ruột của động vật ăn cỏ, nhưng vẫn còn rất ít thông tin về sự đa dạng toàn cầu của chúng. Trong nghiên cứu này, sự đa dạng tập thể của nấm kị khí trong ruột đã được khảo sát bằng cách sử dụng tất cả các trình tự không gian phiên mã nội bộ 1 (ITS1) đã được chỉnh sửa của nấm kị khí trong ruột có sẵn trong GenBank. Có tổng cộng 262.770 trình tự ITS1 nấm đã được kiểm tra chất lượng tải về từ GenBank đã được phân loại thành 274 đơn vị phân loại hoạt động (OTUs) ở mức độ gần tương đương loài. Trong số các OTUs gần tương đương loài này, 119 được đại diện bởi ít nhất năm trình tự ITS1, trong đó có 38 OTUs chứa các loài đã biết và 81 OTUs không chứa loài nào đã biết. Dựa trên phân tích hiếm gặp, các trình tự ITS1 hiện có đại diện cho gần như tất cả các loài chính của nấm kị khí trong ruột, nhưng cần nhiều nỗ lực giải trình tự hơn nữa để đánh giá sự phong phú thực tế của các OTUs nhỏ hơn. Một tập hợp dữ liệu của các trình tự tham chiếu ITS1 (được gọi là AF-RefSeq) và một khung phân loại toàn diện cũng được trình bày, và chúng được cho là thích hợp cho việc phân loại các trình tự ITS1 trong GenBank. Kết quả của nghiên cứu hiện tại có thể giúp hướng dẫn các nghiên cứu trong tương lai liên quan đến phân loại và phân tích phát sinh loài của các trình tự ITS1 của nấm kị khí cũng như việc phân lập và định danh các nấm kị khí mới.
Từ khóa
#nấm kị khí #Neocallimastigomycota #sự đa dạng toàn cầu #phân tích phát sinh loài #phân loạiTài liệu tham khảo
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