Nội dung được dịch bởi AI, chỉ mang tính chất tham khảo
Mạng lưới melamine bimetal nanodendritic palladium-platinum để khuếch đại tín hiệu trong cảm biến voltammetric DNA
Tóm tắt
Một cảm biến DNA điện hóa kiểu sandwich được mô tả nhằm phát hiện các oligonucleotide đặc trưng cho các đột biến gen MECP2. Các hạt nano palladium (PdNPs) và hạt nano platinum (PtNPs) được sử dụng để tổng hợp các nanodendrite PdPt hình hoa bằng phương pháp một nồi. Các nanodendrite PdPt này có diện tích bề mặt riêng lớn và khả năng xúc tác tuyệt vời. Chúng đóng vai trò là chất mang cho probe DNA tín hiệu (SP) và đồng thời xúc tác sự khử hydrogen peroxide (H2O2). Các nanodendrite PdPt được biến đổi với melamine, dẫn đến sự hình thành một mạng lưới PdPt-melamine thông qua các tương tác ổn định giữa các nanodendrite PdPt và ba nhóm amino của mỗi phân tử melamine. Mạng lưới này thể hiện khả năng xúc tác tuyệt vời trong việc tăng cường phản ứng tín hiệu dòng điện trong việc phát hiện đột biến gen MECP2, được đo tốt nhất ở −0.4 V so với SCE và sử dụng H2O2 như là probe điện hóa. Bên cạnh đó, các hoa nano vàng được điện lắng trên giao diện điện cực nhằm tăng tốc độ chuyển electron và bắt giữ probe bắt. Cảm biến này ổn định và có khả năng phát hiện các đột biến gen MECP2 trong khoảng nồng độ từ 1 fmol·L−1 đến 1 nmol·L−1, với giới hạn phát hiện tối thiểu là 0.33 fmol·L−1 tại tỷ lệ S/N bằng 3.
Từ khóa
Tài liệu tham khảo
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