A novel cardiac differentiation method of a large number and uniformly-sized spheroids using microfabricated culture vessels

Shiba, 2016, Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts, Nature, 538, 388, 10.1038/nature19815 Yamamoto, 2016, Electrophysiological characteristics of human iPSC-derived cardiomyocytes for the assessment of drug-induced proarrhythmic potential, PloS One, 11, 10.1371/journal.pone.0167348 Loh, 2016, Mapping the pairwise choices leading from pluripotency to human bone, heart, and other mesoderm cell types, Cell, 166, 451, 10.1016/j.cell.2016.06.011 Yang, 2008, Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population, Nature, 453, 524, 10.1038/nature06894 Kempf, 2014, Controlling expansion and cardiomyogenic differentiation of human pluripotent stem cells in scalable suspension culture, Stem Cell Rep, 3, 1132, 10.1016/j.stemcr.2014.09.017 Kempf, 2015, Cardiac differentiation of human pluripotent stem cells in scalable suspension culture, Nat Protoc, 10, 1345, 10.1038/nprot.2015.089 Pal, 2013, Comparative analysis of cardiomyocyte differentiation from human embryonic stem cells under 3-D and 2-D culture conditions, J Biosci Bioeng, 115, 200, 10.1016/j.jbiosc.2012.08.018 Nguyen, 2014, Microscale generation of cardiospheres promotes robust enrichment of cardiomyocytes derived from human pluripotent stem cells, Stem Cell Rep, 3, 260, 10.1016/j.stemcr.2014.06.002 Ng, 2008, A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies, Nat Protoc, 3, 768, 10.1038/nprot.2008.42 Hwang, 2009, Microwell-mediated control of embryoid body size regulates embryonic stem cell fate via differential expression of WNT5a and WNT11, Proc Natl Acad Sci U S A, 106, 16978, 10.1073/pnas.0905550106 Chen, 2015, Enrichment of cardiac differentiation by a large starting number of embryonic stem cells in embryoid bodies is mediated by the Wnt11-JNK pathway, Biotechnol Lett, 37, 475, 10.1007/s10529-014-1700-5 Mazzotta, 2016, Distinctive roles of canonical and noncanonical Wnt signaling in human embryonic cardiomyocyte development, Stem Cell Rep, 7, 764, 10.1016/j.stemcr.2016.08.008 Sato, 2016, Microfabric vessels for embryoid body formation and rapid differentiation of pluripotent stem cells, Sci Rep, 6, 31063, 10.1038/srep31063 Nakagawa, 2008, Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts, Nat Biotechnol, 26, 101, 10.1038/nbt1374 Kattman, 2011, Stage-specific optimization of activin/nodal and BMP signaling promotes cardiac differentiation of mouse and human pluripotent stem cell lines, Cell stem cell, 8, 228, 10.1016/j.stem.2010.12.008 Birket, 2015, Expansion and patterning of cardiovascular progenitors derived from human pluripotent stem cells, Nat Biotechnol, 33, 970, 10.1038/nbt.3271 Takahashi, 2007, Induction of pluripotent stem cells from adult human fibroblasts by defined factors, Cell, 131, 861, 10.1016/j.cell.2007.11.019 Funakoshi, 2016, Enhanced engraftment, proliferation, and therapeutic potential in heart using optimized human iPSC-derived cardiomyocytes, Sci Rep, 6, 19111, 10.1038/srep19111 Pandur, 2002, Wnt-11 activation of a non-canonical Wnt signalling pathway is required for cardiogenesis, Nature, 418, 636, 10.1038/nature00921 Panakova, 2010, Wnt11 patterns a myocardial electrical gradient through regulation of the L-type Ca(2+) channel, Nature, 466, 874, 10.1038/nature09249 Cohen, 2012, Wnt5a and Wnt11 are essential for second heart field progenitor development, Development, 139, 1931, 10.1242/dev.069377 Gavin, 1990, Expression of multiple novel Wnt-1/int-1-related genes during fetal and adult mouse development, Genes Dev, 4, 2319, 10.1101/gad.4.12b.2319 Yamaguchi, 2008, Genetics of Wnt signaling during early mammalian development, Methods Mol Biol, 468, 287, 10.1007/978-1-59745-249-6_23 Mansson-Broberg, 2016, Wnt/beta-Catenin stimulation and laminins support cardiovascular cell progenitor expansion from human fetal cardiac mesenchymal stromal cells, Stem Cell Rep, 6, 607, 10.1016/j.stemcr.2016.02.014