A novel approach for studying angiogenesis: A human skin equivalent with a capillary-like network

Cell Biology and Toxicology - Tập 15 - Trang 81-90 - 1999
A.F. Black1,2, V. Hudon2, O. Damour1, L. Germain2, F.A. Auger2
1Laboratoire des Substituts Cutanés, CNRS-UPR 412 and Hôpital Edouard Herriot, Lyon, France
2Laboratoire d'Organogénèse Expérimentale/LOEX, CHA pavillon Saint-Sacrement and Département de chirurgie, Faculté de médecine, Université Laval, Québec, Canada

Tóm tắt

Angiogenesis results from an ordered set of events that can be modulated in vivo by a variety of angiogenesis-enhancing or inhibiting agents. We review in vitro angiogenesis models and the agents that enhance or inhibit angiogenesis. We also discuss a new in vitro angiogenesis model created within a skin equivalent. Briefly, endothelial cells were combined with the cutaneous cells of a standard skin equivalent and cultured in a chitosan cross-linked collagen-glycosaminoglycan scaffold of this endothelialized skin. This model enables the formation of capillary-like structures in a coculture environment containing newly synthesized extracellular matrix by fibroblasts and keratinocytes. Several morphological characteristics associated with the microvasculature in vivo were observed in the endothelialized skin equivalent such as histotypic organization of tubular structures, basement membrane deposition, and intercellular junction formation.

Tài liệu tham khảo

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