A nanoemulsion-based nanogel of Citrus limon essential oil with leishmanicidal activity against Leishmania tropica and Leishmania major

Journal of Parasitic Diseases - Tập 45 - Trang 441-448 - 2020
Elham Zarenezhad1, Mahmoud Agholi2, Ali Ghanbariasad3, Ali Ranjbar4, Mahmoud Osanloo5
1Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
2Department of Medical Parasitology and Mycology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
3Department of Medical Biotechnology, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
4Department of Medicine, School of Medicine, Fasa University of Medical Sciences, Fasa, Iran
5Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Fasa University of Medical Sciences, Fasa, Iran

Tóm tắt

Cutaneous leishmaniasis is one of the diseases that severely affects human skin. Nanogels are the well-known formulation for topical drug delivery due to easy usage, high loading capacity, and physical and chemical stabilities. In this study, the toxicity effect of three essential oils, including Mentha piperita, Anethum graveolens, and Citrus limon (CLEO), was evaluated against Leishmania major and Leishmania tropica. Ingredients of CLEO as the most potent essential oil were identified using GC–MS analysis. The five major components were limonene (61.83%), sabinene (16.99%), trans-limonene oxide (3.08%), cis-limonene oxide (2.27%), and 1,2-cyclohexane diol, 1-methyl-4-(1-methylethenyl) (1.50%). The nanogel of CLEO (CLNgel) was prepared by the addition of carbomer 940 (1% w/v) to the prepared nanoemulsion with a droplet size of 146 ± 12 nm. The viscosity of CLNgel was fitted with a regression of non-Newtonian materials, Carreau-Yasuda. Interestingly, CLNgel at a concentration of 80 µg/mL reduced the viability of both species to 0%. Therefore, the prepared prototype can/could/would be used as an excellent nanoformulation for in vivo studies.

Tài liệu tham khảo

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