A multilayer scaffold design with spatial arrangement of cells to modulate esophageal tissue growth

Journal of Biomedical Materials Research - Part B Applied Biomaterials - Tập 107 Số 2 - Trang 324-331 - 2019
Sherif Soliman1,2, J Laurent1,2, Lena Kalenjian1, KaLia S. Burnette1, Bert Hedberg1, Saverio La Francesca1
1Biostage Inc, Holliston, Massachusetts, 01746
2these authors contributed equally to this work

Tóm tắt

AbstractEsophageal diseases may require resectioning of the damaged portion. The current standard of care requires the replacement of the esophagus with the stomach or the intestine. Such procedures have high rates of mortality and morbidity; therefore, the use of alternative conduits is needed. A tissue engineering approach that allows for the regeneration of esophageal tissues would have significant clinical application. A cell‐seeded synthetic scaffold could replace the resected part of the esophagus and elicit tissue regrowth. In order to ideally recreate a functioning esophagus, its two crucial tissue layers should be induced: an epithelium on the luminal surface and a muscle layer on the exterior surface. To create a bioengineered esophagus with both tissue layers, a multilayer (ML) tubular scaffold design was considered. Luminal and exterior layers were electrospun with broad pore size to promote penetration and proliferation of mesenchymal stem cells on the lumen and smooth muscle cells on the external. These two layers would be separated by a thin layer with substantially narrower pore size intended to act as a barrier for the two cell types. This ML scaffold design was achieved via electrospinning by tuning the solution and the process parameters. Analysis of the scaffold demonstrated that this tuning enabled the production of three integrated layers with distinguishable microstructures and good mechanical integrity. In vitro validation was conducted on the separated unilayer components of the ML scaffold. The resultant proof‐of‐concept ML scaffold design could possibly support the spatial arrangement of cells needed to promote esophageal tissue regeneration. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 324–331, 2019.

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Journal of Biomedical Materials Research - Part B Applied Biomaterials

Tập 107 Số 2

324-331

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