A microfluidic device for capturing malaria-infected red blood cells by magnetophoretic force using an array of V- and W-shaped nickel microstructures

Microfluidics and Nanofluidics - Tập 26 - Trang 1-13 - 2022
Pachara Noosawat1, Werayut Srituravanich1, Nattapol Damrongplasit1, Yuji Suzuki2, Morakot Kaewthamasorn3, Alongkorn Pimpin1
1Micro/Nano Electromechanical Integrated Device Research Unit, Department of Mechanical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
2Department of Mechanical Engineering, The University of Tokyo, Tokyo, Japan
3Veterinary Parasitology Research Unit, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand

Tóm tắt

A microfluidic device with ferromagnetic microstructures designed to locally induce strong magnetophoretic force for capturing infected blood cells has been employed for malaria diagnosis for a long time. In this study, new configurations of V- and W-shaped nickel microstructures fabricated by an electroplating process and magnet arrays were proposed to enhance the capture efficacy from the conventional square one. The simulation of magnetophoretic and hydrodynamic forces was conducted to reveal the capture mechanism of them. After that, the microfluidic device was built and tested. The experimental results with magnetic beads showed that capture efficiency increased by almost twice as much when compared to the conventional square microstructures. Additionally, malaria-infected blood cells at the level of parasitemia at 2%, 10%, and 80% were tested, and the capture efficiency was in agreement with the tests with magnetic beads.

Tài liệu tham khảo

Abedini-Nassab R (2019) Magnetomicrofluidic platforms for organizing arrays of single-particles and particle-pairs. J Microelectromech Syst 28:4. https://doi.org/10.1109/JMEMS.2019.2912649 Aslan G, Seyrek A, Kocagoz T, Ulukanlıgil M, Erguven S, Gunalp A (2007) The diagnosis of malaria and identification of plasmodium species by polymerase chain reaction in Turkey. Parasitol Int 56:217–220 Atkinson CT, Aikawa M (1990) Ultrastructure of malaria-infected erythrocytes. Blood Cells 16:351–368 Azikiwe CCA, Ifezulike CC, Siminialayi IM, Amazu LU, Enye JC, Nwakwunite OE (2012) A comparative laboratory diagnosis of malaria: microscopy versus rapid diagnostic test kits. Asian Pac J Trop Biomed 2:307–310 Butykai A, Orban A, Kocsis V, Szaller D, Bordacs S, Tatrai-Szekeres E, Kiss LF, Bota A, Vertessy BG, Zelles T, Kezsmarki I (2013) Malaria pigment crystals as magnetic micro-rotors: key for high-sensitivity diagnosis. Sci Rep 3:1431. https://doi.org/10.1038/srep01431 Chen P, Huang Y, Hoshino K, Zhang J (2015) Microscale magnetic field modulation for enhanced capture and distribution of rare circulating tumor cells. Sci Rep 5:08745. https://doi.org/10.1038/srep08745 Chen H, Zhang Z, Liu H, Zhang Z, Lin C, Wang B (2019) Hybrid magnetic and deformability-based isolation of circulating tumor cells using microfluidics. AIP Adv 9:025023. https://doi.org/10.1063/1.5081849 Earhart CM, Wilson RJ, White RL, Pourmand N, Wang SX (2009) Microfabricated magnetic sifter for high-through and high-gradient magnetic separation. J Magnet Magnet Mater 321:1436–1439 Gascoyne P, Satayavivad J, Ruchirawat M (2004) Microfluidic approaches to malaria detection. Acta Trop 89:357–369 Gascoyne P, Mahidol C, Ruchirawat M, Satayavivad J, Watcharasit P, Becker F (2002) Microsample preparation by dielectrophoresis: Isolation of malaria. Lab Chip 2:70–75 Grover WH, Bryan AK, Silva MD, Suresh S, Higgins JM, Manalis SR (2011) Measuring single-cell density. PNAS 108:10992–10996 Guo Q, Duffy SP, Matthews K, Deng X, Santoso AT, Islamzada E, Ma H (2016) Deformability based sorting of red blood cells improves diagnostic sensitivity for malaria caused by Plasmodium falciparum. Lab Chip 16:645–654 Hanschied T, Calvalho T, Grobusch MP (2016) Hemozoin detection for human malaria diagnosis investigated in rodent models: How similar is similar? Trends Parasitol 32:26704075. https://doi.org/10.1016/j.pt.2015.11.012 Huang NT, Hwong YJ, Lai RL (2018) A microfluidic microwell device for immunomagnetic single-cell trapping. Microfluidics Nanofluidics 22:16. https://doi.org/10.1007/s10404-018-2040-x Kasetsirikul S, Srituravanich W, Piyaviriyakul P, Pimpin A (2017) Separation of magnetic particles using an array of magnets – a model of a separation device for malaria-infected blood cells. Sensors Mater 29:281–291 Kasetsirikul S, Buranapong J, Srituravanich W, Kaewthamasorn M, Pimpin A (2016) The development of malaria diagnostic techniques: a review of the approaches with focus on dielectrophoretic and magnetophorectic methods. Malaria J 15:358. https://doi.org/10.1186/s12936-016-1400-9 Kim J, Kim CN (2015) Evaluation of optimization algorithms for the design of a magnetic cell sorter for malaria-infected blood. J Mech Sci Technol 29:4833–4839 Kim J, Massoudi M, Antaki JF, Gandini A (2012) Removal of malaria-infected red blood cells using magnetic cell separators: a computational study. Appl Math Comput 218:6841–6850 Kolluri N, Klapperich CM, Cabodi M (2018) Towards lab-on-a-chip diagnostics for malaria elimination. Lab Chip 18:75–94 Kotepui M, Piwkham D, Phunphuech B, Phiwklam N, Chupeerach C, Duangmano S (2015) Effects of malaria parasite density on blood cell parameters. PLoS ONE. https://doi.org/10.1371/journal.pone.0121057 Krampa FD, Aniweh Y, Kanyong P, Awandare G (2020) Recent advances in the development of biosensors for malaria diagnosis. Sensors 20:799. https://doi.org/10.3390/s20030799 Kyabayinze DJ, Tibenderana JK, Odong GW, Rwakimari JB, Counihan H (2008) Operational accuracy and comparative persistent antigenicity of HRP2 rapid diagnostic tests for Plasmodium falciparum malaria in a hyperendemic region of Uganda. Malaria J 7:221. https://doi.org/10.1186/1475-2875-7-221 Martin AB, Wu WT, Kameneva MV, Antaki JF (2017) Development of a high-throughput magnetic separation device for malaria-infected erythrocytes. Ann Biomed Eng 45:2888–2898 McMorrow LM, Masanja MI, Kahigwa E, Abdulla SMK, Kachur SP (2010) Quality assurance of rapid diagnostic tests for malaria in routine patient care in rural Tanzania. Am J Trop Med Hyg 82:151–155 Moody A (2002) Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 1:66–78 Morassin B, Fabre R, Berry A, Magnaval JF (2002) One year’s experience with the polymerase chain reaction as a routine method for the diagnosis of imported malaria. Am J Trop Med Hyg 66:503–508 Nam J, Huang H, Lim H, Lim C, Shin S (2013) Magnetic separation of malaria-infected red blood cells in various developmental stages. Anal Chem 85:7316–7323 Nash GB, O’Brien E, Gordon-Smith EC, Dormandy JA (1989) Abnormalities in the mechanical properties of red blood cells caused by plasmodium falciparum. Blood 74:855–861 Pham NM, Karlen W, Beck HP, Delamarche E (2018) Malaria and the last parasite: how can technology help? Malaria J 17:260. https://doi.org/10.1186/s12936-018-2408-0 Postigo M, Mendoza-Leon A, Perez HA (1998) Malaria diagnosis by the polymerase chain reaction: a field study in south-eastern Venezuela. Trans R Soc Trop Med Hyg 92:509–511 Sakaguchi M, Miyazaki N, Fujioka H, Kaneko O, Murata K (2016) Three-dimensional analysis of morphological changes in the malaria parasite infected red blood cell by serial block-face scanning electron microscopy. J Struct Biol 193:162–171 Snounou G, Viriyakosol S, Jarra W, Thaithong S, Brown KN (1993) Identification of the four human malaria parasite species in field samples by the polymerase chain reaction and detection of a high prevalence of mixed infections. Mol Biochem Parasitol 58:283–292 Sumari D, Grimberg BT, Blankenship DA, Mugasa J, Mugittu K, Moore L, Gwakisa P, Zborowski M (2016) Application of magnetic cytosmear for the estimation of Plasmodium falciparum gametocyte density and detection of asexual stages in asymptomatic children. Malaria J 15:113. https://doi.org/10.1186/s12936-016-1170-4 Tangpukdee N, Duangdee C, Wilairatana P, Krudsood S (2009) Malaria diagnosis: a brief review. Korean J Parasitol 47:93–102 World Health Organization (2015) Malaria. http://www.who.int/topics/malaria/en/. Accessed Jan 2019 Wu J, Cui Y, Xuan S, Gong X (2018) 3D-printed microfluidic manipulation device integrated with magnetic array. Microfluidics Nanofluidics 22:103. https://doi.org/10.1007/s10404-018-2123-8 Wu WT, Martin AB, Gandini A, Aubry N, Massoudi M, Antaki JF (2016) Design of microfluidic channels for magnetic separation of malaria-infected red blood cells. Microfluid Nanofluid 20:41. https://doi.org/10.1007/s10404-016-1707-4 Xia J, Chen X, Zhou CZ, Li YG, Peng ZH (2011) Development of a low-cost magnetic microfluidic chip for circulating tumor cell capture. IET Nanobiotechnol 5:114–120 Yang X, Chen Z, Miao J, Cui L, Guan W (2017) High-throughput and label-free parasitemia quantification and stage differentiation for malaria-infected red blood cells. Biosensors Bioelectronics 98:408–414 Yu X, Wen CY, Zhang ZL, Pang DW (2014) Control of magnetic field distribution by using nickel powder-PDMS pillars in microchannels. RSC Adv 4:17660–17666 Zhou Y, Wang Y, Lin Q (2010) A microfluidic device for continuous-flow magnetically controlled capture and isolation of microparticles. J Microelectromech Syst 19:743–751