A microchip to analyze single crystal growth and size-controllability
Tóm tắt
A microfluidic device to control single crystallization on the micron scale has been developed. The salt solution was stored in the nano-volume gaps between the arrays of protrudent circular plots in the microchip. The mixed organic solvent was injected into the chip as the counter diffusion phase for crystallization forming. This device provides a liquid-liquid interface through which only one phase flows while the other stays at the fixed plot. Therefore, it is possible to control the position of crystallization on the fixed plot. We can control the size and the uniformity of single crystals from 5 to 50 μm in length by adjusting the relative factors, such as interface lifetime, breeds of the mix-organic solvents and injecting velocities. The longer interface lifetime and lower organic solvent injecting velocities can bring up larger and more asymmetric crystals, which nearly shows the same trend compared with the macroscopic crystallization. Finally, the effect of the surfactant on the crystallization in the micro-device was studied. By adding the surfactant into the liquid-liquid interface, smaller sizes of crystals can be obtained without changing the crystal configuration.
Tài liệu tham khảo
Bolanos-Garcia V M. The use of oil in a counter-diffusive system allows to control nucleation and coarsening during protein crystallization. J Cryst Growth, 2003, 253: 517–523
Henrista C, Mathieua J P, Vogelsb C, Rulmonta A, Cloots R. Morphological study of magnesium hydroxide nanoparticles precipitated in dilute aqueous solution. J Cryst Growth, 2003, 249: 321–330
Hansen C L, Classen S, Berger J M, Quake S R. A microfluidic device for kinetic optimization of protein crystallization and in situ structure determination. J Am Chem Soc, 2006, 128: 3142–3143
Leng J, Lonetti B, Tabeling P. Microevaporators for kinetic exploration of phase diagrams. Phys Rev Lett, 2006, 96: 084503-1–084503-4
Gerdts C J, Tereshko V, Yadav M K, Dementieva I, Collart F, Joachimiak A, Stevens R C, Kuhn P, Kossiakoff A, Ismagilov R F. Time-controlled microfluidic seeding in nL-volume droplets to separate nucleation and growth stages of protein crystallization. Angew Chem Int Ed, 2006, 45: 8156–8160
Shim J U, Cristobal G, Link D R, Thorsen T, Jia Y W, Piattelli K, Fraden S. Control and measurement of the phase behavior of aqueous solutions using microfluidics. J Am Chem Soc, 2007, 129: 8825–8835
Garcia-Ruiz J M, Novella M L, Otalora F J. Supersaturation patterns in counter-diffusion crystallization methods followed by Mach-Zehnder interferometry. J Cryst Growth, 1999, 196: 703–710
Kenis P J A, Ismagilov R F, Whitesides G M. Microfabrication inside capillaries using multiphase laminar flow patterning. Science, 1999, 285: 83–85
Laval P, Salmon J B, Joanicot M. A microfluidic device for investigating crystal nucleation kinetics, J Cryst Growth, 2007, 303: 622–628
Hansen C, Quake S R. Microfluidics in structural biology: Smaller, faster ... better. Curr Opin Struct Biol, 2003, 13: 538–544
Howell T A, Eyal B Y, Rao C, Hartel R W. Sucrose crystallization kinetics in thin films at elevated temperatures and supersaturations. Cryst Growth Des, 2002, 2: 67–72
Aizenberg J, Black A J, Whitesides G M. Control of crystal nucleation by patterned self-assembled monolayers. Nature, 1999, 398: 495–498
Liu J J, Lin J M. Measurements of surface tension of organic solvents using a simple microfabricated chip. Anal Chem, 2007, 79: 371–377
Atencia J, Beebe D J. Steady flow generation in microcirculatory systems. Lab Chip, 2006, 6: 567–574
Park J U, Meitl M A, Hur S H, Usrey M L, Strano M S, Kenis P J A, Roger J A. In situ deposition and patterning of single-walled carbon nanotubes by laminar flow and controlled flocculation in microfluidic channels. Angew Chem Int Ed, 2006, 45: 581–585
Yang M S, Yang J, Li C W, Zhao J L. Generation of concentration gradient by controlled flow distribution and diffusive mixing in a microfluidic chip. Lab Chip, 2002, 2: 158–163
Wang Z, Chen J F, Le Y, Shen Z G. Preparation of ultrafine beclomethasone dipropionate drug powder by antisolvent precipitation. Ind Eng Chem Res, 2007, 46: 4839–4845
Auroux P A, Iossifidis D, Reyes D R, Manz A. Micro total analysissystems. 2. Analytical standard operations and applications. Anal Chem, 2002, 74: 2637–2652
Grohe B, Young J O, Ionescu D A, Lajoie G, Rogers K A, Karttunen M, Goldberg H A, Hunter G K. Control of calcium oxalate crystal growth by face-specific adsorption of an osteopontin phosphopeptide. J Am Chem Soc, 2007, 129: 14946–14951
Shinohara K, Fukui T, Abe H, Sekimura N, Okamoto K. Screening of C60 crystallization using a microfluidic system. Langmuir, 2006, 22:6477–6480