A <i>Sod2</i> Null Mutation Confers Severely Reduced Adult Life Span in Drosophila

Genetics - Tập 165 Số 4 - Trang 2295-2299 - 2003
Atanu Duttaroy1, Anirban Paul1, Mukta Kundu1, Amy Belton1
1Biology Department, Howard University, Washington, DC 20059

Tóm tắt

Abstract A null mutation for the Sod2 gene, Sod2n283, was obtained in Drosophila melanogaster. Homozygous Sod2 null (Sodn283/Sodn283) adult flies survive up to 24 hr following eclosion, a phenotype reminiscent of mice, where Sod2–/– progeny suffer neonatal lethality. Sodn283/+ heterozygotes are sensitive to oxidative stress induced by paraquat treatment.

Từ khóa


Tài liệu tham khảo

Duttaroy, 1994, A manganese superoxide dismutase-encoding cDNA from Drosopohila melanogaster, Gene, 143, 223, 10.1016/0378-1119(94)90100-7

Duttaroy, 1997, The manganese superoxide dismutase gene of Drosophila: structure, expression and evidence for regulation by MAP kinase, DNA Cell Biol., 16, 391, 10.1089/dna.1997.16.391

FlyBase, 1999, The FlyBase database of the Drosophila Genome Projects and community literature, Nucleic Acids Res., 27, 85, 10.1093/nar/27.1.85

Kirby, 2002, RNA interference-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress, Proc. Natl. Acad. Sci. USA, 99, 16162, 10.1073/pnas.252342899

Kokoszka, 2000, Increased mitochondrial oxidative stress in the Sod2(+/–) mouse results in the age-related decline of mitochondrial function culminating in increased apoptosis, Proc. Natl. Acad. Sci. USA, 98, 2278, 10.1073/pnas.051627098

Lebovitz, 1996, Neurodegeneration, myocardial injury, and perinatal death in mitochondrial superoxide dismutase-deficient mice, Proc. Natl. Acad. Sci. USA, 93, 9782, 10.1073/pnas.93.18.9782

Li, 1995, Dilated cardiomyopathy and neonatal lethality in mutant mice lacking manganese superoxide dismutase, Nat. Genet., 11, 376, 10.1038/ng1295-376

Melov, 1999, Mitochondrial disease in superoxide dismutase 2 mutant mice, Proc. Natl. Acad. Sci. USA, 96, 846, 10.1073/pnas.96.3.846

Mockett, 1999, Overexpression of Mn-containing superoxide dismutase in transgenic Drosophila melanogaster, Arch. Biochem. Biophys., 371, 260, 10.1006/abbi.1999.1460

Phillips, 1989, Null mutation for copper/zinc superoxide dismutase in Drosophila confers hypersensitivity to paraquat and reduced longevity, Proc. Natl. Acad. Sci. USA, 86, 2761, 10.1073/pnas.86.8.2761

Phillips, 1995, Subunit-destabilizing mutations in Drosophila copper/zinc superoxide dismutase: neuropathology and a model for dimmer disequilibrium, Proc. Natl. Acad. Sci. USA, 92, 8574, 10.1073/pnas.92.19.8574

Reaume, 1996, Motor neurons in Cu/Zn superoxide dismutase-deficient mice develop normally but exhibit enhanced cell death after axonal injury, Nat. Genet., 13, 43, 10.1038/ng0596-43

Robertson, 1988, A stable genomic source of transposase in Drosophila melanogaster, Genetics, 118, 461, 10.1093/genetics/118.3.461

Rogina, 2000, Cu, Zn superoxide dismutase deficiency accelerates the time course of an age-related marker in Drosophila melanogaster, Biogerontology, 1, 163, 10.1023/A:1010039813107

Roseman, 1995, A P element containing suppressor of hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster, Genetics, 141, 1061, 10.1093/genetics/141.3.1061

Sun, 2002, Induced overexpression of mitochondrial Mn-superoxide dismutase extends the life span of adult Drosophila melanogaster, Genetics, 161, 661, 10.1093/genetics/161.2.661

Wallace, 2001, Mouse models for mitochondrial disease, Am. J. Med. Genet., 106, 71, 10.1002/ajmg.1393