A homolog of splicing factor SF1 is essential for development and is involved in the alternative splicing of pre‐mRNA in Arabidopsis thaliana

Plant Journal - Tập 78 Số 4 - Trang 591-603 - 2014
Yun Hee Jang1, Hyo‐Young Park1, Keh Chien Lee1, May Phyo Thu1, Soon‐Kap Kim1, Mi Chung Suh2, Hunseung Kang3, Jeong‐Kook Kim1
1Plant Signaling Network Research Center, School of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea
2Department of Bioenergy Science and Technology; Chonnam National University; Gwangju 500-757 Korea
3Division of Plant Biotechnology; Chonnam National University; Gwangju 500-757 Korea

Tóm tắt

Summary

During initial spliceosome assembly, SF1 binds to intron branch points and interacts with U2 snRNP auxiliary factor 65 (U2AF65). Here, we present evidence indicating that AtSF1, the Arabidopsis SF1 homolog, interacts with AtU2AF65a and AtU2AF65b, the Arabidopsis U2AF65 homologs. A mutant allele of AtSF1 (At5g51300) that contains a T–DNA insertion conferred pleiotropic developmental defects, including early flowering and abnormal sensitivity to abscisic acid. An AtSF1 promoter‐driven GUS reporter assay showed that AtSF1 promoter activity was temporally and spatially altered, and that full AtSF1 promoter activity required a significant proportion of the coding region. DNA chip analyses showed that only a small proportion of the transcriptome was altered by more than twofold in either direction in the AtSF1 mutant. Expression of the mRNAs of many heat shock proteins was more than fourfold higher in the mutant strain; these mRNAs were among those whose expression was increased most in the mutant strain. An RT–PCR assay revealed an altered alternative splicing pattern for heat shock transcription factor HsfA2 (At2g26150) in the mutant; this altered splicing is probably responsible for the increased expression of the target genes induced by HsfA2. Altered alternative splicing patterns were also detected for the transcripts of other genes in the mutant strain. These results suggest that AtSF1 has functional similarities to its yeast and metazoan counterparts.

Từ khóa


Tài liệu tham khảo

10.1016/S0092-8674(00)80221-4

10.1093/pcp/pcr077

10.1104/pp.104.058958

10.1111/j.1365-313X.2006.02802.x

Arning S., 1996, Mammalian splicing factor SF1 is encoded by variant cDNAs and binds to RNA, RNA, 2, 794

10.1101/gr.053678.106

10.1016/S0092-8674(00)80261-5

10.1111/j.1365-313X.2004.02194.x

10.1111/j.1365-313X.2004.02206.x

10.1104/pp.110.155523

10.2307/3870759

10.1104/pp.109.141705

10.1126/science.1069415

10.1046/j.1365-313x.1999.00623.x

10.1093/nar/gkq1042

10.1093/nar/gkm569

10.1016/S0092-8674(03)01026-2

10.1074/jbc.M603137200

10.1074/jbc.M609430200

10.1111/j.1365-313X.2005.02473.x

10.1016/j.cell.2009.01.019

10.1016/S0092-8674(01)00460-3

10.1007/s12374-009-9018-x

10.1093/pcp/pcp093

10.1101/gad.1206204

10.1105/tpc.110.079103

10.1104/pp.105.070318

10.1111/j.1365-313X.2007.03110.x

10.1038/sj.emboj.7600121

10.1007/s11103-005-8105-8

10.1105/tpc.106.042184

10.1128/MCB.24.20.9176-9185.2004

10.1093/nar/30.3.623

10.1016/S1360-1385(00)01595-8

10.1017/S1355838299991872

10.1016/j.tibs.2008.06.001

10.1111/j.1365-313X.2008.03650.x

10.1093/pcp/pcq038

10.1371/journal.ppat.1000526

10.1111/j.1365-313X.2006.02889.x

10.1105/tpc.113.111922

10.1073/pnas.0407004101

10.1105/tpc.112.103697

10.1371/journal.pbio.0050090

10.1105/tpc.13.6.1263

10.1017/S1355838298980335

10.1146/annurev.arplant.58.032806.103754

Ru Y., 2008, Spliceosomal proteins in plants, Curr. Top. Microbiol. Immunol., 326, 1

10.1017/S1355838299982286

10.1093/emboj/19.8.1873

10.1074/jbc.M408815200

10.1101/gr.6818908

10.1111/j.1349-7006.2007.00629.x

10.1105/tpc.108.062596

10.1105/tpc.110.074674

10.1091/mbc.E04-11-1034

10.1016/j.jmb.2005.11.067

10.1007/978-0-387-39975-1_9

10.1016/j.cell.2009.02.009

10.1186/gb-2004-5-12-r102

10.1073/pnas.0602039103

10.1093/nar/gkn144

10.1093/emboj/20.16.4536

10.1126/science.1167222

10.1016/S1534-5807(01)00087-9

10.1093/pcp/pcp011

10.1104/pp.109.138180

10.1105/tpc.110.081356

10.1038/emboj.2013.49

Thông tin
Thông tin xuất bản

Plant Journal

Tập 78 Số 4

591-603

Thông tin tác giả