A dual-cross-linked hydrogel based on hyaluronic acid/gelatin tethered via tannic acid: mechanical properties’ enhancement and stability control

Iranian Polymer Journal - 2021
Shan Yu1,2, Yuxing Ji1,2, Cuiping Guo1,2, Daohuan Lu1,2, Zhijie Geng1,2, Dating Pei1,2, Qunfeng Liu1,2
1National Engineering Research Center for Healthcare Devices, Guangzhou, China
2Guangdong Key Lab of Medical Electronic Instruments and Polymer Material Products, Guangdong Institute of Medical Instruments, Guangzhou, China

Tóm tắt

Extracellular matrix (ECM) as a network is mainly composed of glycosaminoglycans and proteins. Among these glycosaminoglycans and proteins that naturally occur in ECM, hyaluronic acid (HA) and gelatin attract more attentions. In this work, a chemically cross-linked HA/gelatin hydrogel (HG hydrogel) was firstly fabricated, and then, tannic acid (TA) was introduced as a physical cross-linker, to form a dual-cross-linked network (HG-TAx hydrogels). The strong hydrogen bonding between TA and HG hydrogel resulted in lower swelling ratio, decreasing from 220% (pristine HG hydrogels) to 7.5% (HG-TA25 hydrogels) and stronger mechanical properties increasing from 6 kPa (HG hydrogel) to 160 kPa (HG-TA25 hydrogel). The hydrogel stability in enzyme was significantly improved, attributing to the hyaluronidase inhibition activity of TA. The degradation time significantly increased along with the addition of TA (from 7 up to 21 days). Furthermore, the HG-TAx hydrogels exhibited good cleavage ability to reactive oxygen species (ROS) that could be generated in human tissues, and the antioxidant capacity increased up to 24.2 mg/mL of L-ascorbic acid standard. The improved mechanical properties, prolonged degradation time, and potential antioxidant ability of the HG-TAx hydrogels could pave the way for the design of biomaterials. Furthermore, TA has shown potential biological functions properties and brought the great potential of the HG-TAx hydrogels for implant applications.

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