A cyclic dinucleotide containing 2-aminopurine is a general fluorescent sensor for c-di-GMP and 3′,3′-cGAMP

Royal Society of Chemistry (RSC) - Tập 10 Số 6 - Trang 1568-1575
Benjamin T. Roembke1,2,3, Jie Zhou1,2,3, Yue Zheng1,2,3, David Sayre1,2,3, Allan Lizardo1,2,3, Laurentee Bernard1,2,3, Herman O. Sintim1,2,3
1College Park, USA
2Department of Chemistry and Biochemistry, University of Maryland, College Park, USA
3University of Maryland

Tóm tắt

A cyclic dinucleotide containing 2-amino purine is fluorescent but upon dimer formation with c-di-GMP or cGAMP, in the presence of Mn2+, the fluorescence of the 2AP probe is quenched, providing a means to detect c-di-GMP.

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