A convenient technique for the dissection of mosquito salivary glands

A method is described wherebyP. berghei sprorozoites may be obtained from the salivary glands of infected femaleA. stephensi mosquitoes. The method is relatively easy to master, requires minimal handling of biological material, and is done with ordinary laboratory equipment. In addition, the sporozoites are rendered relatively free of contaminating micro-organisms and the entire operation may be performed aseptically.