A complete backbone spectral assignment of lipid-free human apolipoprotein E (apoE)

Biomolecular NMR Assignments - Tập 2 - Trang 207-210 - 2008
Yonghong Zhang1, Jianglei Chen1, Jianjun Wang1
1Department of Biochemistry and Molecular Biology, School of Medicine, Wayne State University, Detroit, USA

Tóm tắt

Apolipoprotein E is an exchangeable apolipoprotein that plays an important role in lipid/lipoprotein metabolism and cardiovascular diseases. Recent evidence indicates that apoE is also critical in several other important biological processes, including Alzheimer’s disease, cognitive function, immunoregulation, cell signaling and infectious diseases. Although the X-ray crystal structure of the apoE N-terminal domain was solved in 1991, there is no structure available for the apoE C-terminal domain and full-length apoE. Here we report a complete NMR backbone spectral assignment of lipid-free human apoE.

Tài liệu tham khảo

Cornilescu G, Delaglio F, Bax A (1999) Protein backbone angle restraints from searching a database for chemical shift and sequence homology. J Biomol NMR 13(3):289–302. doi:10.1023/A:1008392405740 Corder EH, Saunders AM, Strittmatter WJ, Schmechel DE, Gaskell PC (1993) Gene dose of apolipoprotein E type 4 allele and the risk of Alzheimer’s disease in late onset families. Science 261:921–923. doi:10.1126/science.8346443 Dong LM, Weisgraber KH (1996) Human apolipoprotein E4 domain interaction Arginine 61 and glutamic acid 255 interact to direct the preference for very low density lipoproteins. J Biol Chem 271:19053–19057. doi:10.1074/jbc.271.32.19053 Eghbalnia HR, Wang L, Bahrami A, Assadi A, Markley JL (2005) Protein energetic conformational analysis from NMR chemical shifts (PECAN) and its use in determining secondary structural elements. J Biomol NMR 32:71–81. doi:10.1007/s10858-005-5705-1 Fan D, Li Q, Korando L, Jerome WG, Wang J (2004) A monomeric human apolipoprotein E carboxyl-terminal domain. Biochemistry 43:5055–5064. doi:10.1021/bi035958w Huang Y, von Eckardstein A, Wu S, Maeda N, Assmann G (1994) A plasma lipoprotein containing only apolipoprotein E and with gamma mobility on electrophoresis releases cholesterol from cells. Proc Natl Acad Sci USA 91:1834–1838. doi:10.1073/pnas.91.5.1834 Mahley RW, Rall SC Jr (2000) Apolipoprotein E: far more than a lipid transport protein. Annu Rev Genomics Hum Genet 1:507–537. doi:10.1146/annurev.genom.1.1.507 Mahley RW, Huang Y (1999) Apolipoprotein E: from atherosclerosis to Alzheimer’s disease and beyond. Curr Opin Lipidol 10:207–217. doi:10.1097/00041433-199906000-00003 Peters-Libeu CA, Newhouse Y, Hatters DM, Weisgraber KH (2006) Model of biologically active apolipoprotein E bound to dipalmitoylphosphatidylcholine. J Biol Chem 281:1073–1079. doi:10.1074/jbc.M510851200 Schneider WJ, Kovanen PT, Brown MS, Goldstein JL, Utermann G (1981) Familial dysbetalipoproteinemia. Abnormal binding of mutant apoprotein E to low density lipoprotein receptors of human fibroblasts and membranes from liver and adrenal of rats, rabbits, and cows. J Clin Invest 68:1075–1085. doi:10.1172/JCI110330 Sivashanmugam A, Victoria Meiners, Chunxian Cui, Yunhuang Yang, Jianjun Wang, Qianqian Li (2008) Practical protocols for production of very high-yield of recombinant proteins in Eschericia coli. Submitted to Protein Sci Weisgraber KH (1994) Apolipoprotein E: structure-function relationships. Adv Protein Chem 45:249–302. doi:10.1016/S0065-3233(08)60642-7 Wilson C, Wardell MR, Weisgraber KH, Mahley RW, Agard DA (1991) Three-dimensional structure of the LDL receptor-binding domain of human apolipoprotein E. Science 252:1817–1822. doi:10.1126/science.2063194 Wishart DS, Sykes BD, Richards FM (1992) The chemical shift index: a fast and simple method for the assignment of protein secondary structure through NMR spectroscopy. Biochemistry 31:1647–1651. doi:10.1021/bi00121a010 Xu C, Sivashanmugam A, Hoyt D, Wang J (2005) A complete backbone assignment of the human apolipoprotein E LDL-receptor-binding domain. J Biomol NMR 32:177. doi:10.1007/s10858-005-6729-2 Zhang Y, Vasudevan S, Sojitrawala R, Zhao W, Cui C, Xu C et al (2007) A monomeric, biologically active, full-length human apolipoprotein E. Biochemistry 46:10722–10732. doi:10.1021/bi700672v Zhao W, Zhang Y, Cui C, Li Q, Wang J (2008) An efficient on-column expressed protein ligation strategy: application to segmental triple labeling of human apolipoprotein E3. Protein Sci 17:736–747. doi:10.1110/ps.073383708