Nghiên cứu so sánh phương pháp PAS-phosphotungstic acid-Diamine Supra Blue FGL và phản ứng miễn dịch hóa cho collagen loại I

Springer Science and Business Media LLC - Tập 91 - Trang 283-289 - 1989
R. N. Rao1, P. M. Fallman1, D. Greer Falls1, S. N. Meloan1
1Department of Pathology, Medical College of Georgia, Augusta, USA

Tóm tắt

Các kỹ thuật miễn dịch hóa mô đã chứng minh giá trị của chúng trong các nghiên cứu mô học về các loại collagen khác nhau. Tuy nhiên, những điểm hạn chế bao gồm phản ứng không đặc hiệu của các kháng thể, việc che lấp các kháng nguyên, và chi phí cao cho các kháng thể. Nghiên cứu này được thực hiện nhằm xác định tính đặc hiệu của phản ứng PAS-phosphotungstic acid-Diamine Supra Blue FGL (PAS PTA-DSB-FGL) đối với collagen loại I, phân biệt nó với các loại collagen khác. Các chuỗi lặp lại của các mẫu cắt mô cố định bằng methaearn từ các mô khác nhau đã được xử lý bằng phản ứng PAS-PTA-DSB FGL và kỹ thuật peroxidase-antiperoxidase (PAP) cho collagen loại I, và các mô hình nhuộm đã được so sánh. Các sợi gắn thuốc nhuộm màu xanh chỉ được tìm thấy tại những vị trí phản ứng với các kháng thể chống lại collagen loại I. Những quan sát này cho thấy quy trình PAS-PTA-DSB FGL là phù hợp cho việc visual hóa collagen loại I, ví dụ, trong việc sàng lọc một loạt lớn các mẫu cắt và trong thực hành bệnh lý phẫu thuật và khám nghiệm tử thi.

Từ khóa

#collagen loại I #PAS-phosphotungstic acid-Diamine Supra Blue FGL #kỹ thuật miễn dịch hóa mô #bệnh lý phẫu thuật #khám nghiệm tử thi

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