A compact fluorescence polarization analyzer with high-transmittance liquid crystal layer

Review of Scientific Instruments - Tập 89 Số 2 - 2018
Osamu Wakao1, Ken Satou2, Ayano Nakamura3, Ken Sumiyoshi2, Masanori Shirokawa2, Chikaaki Mizokuchi2, Kunihiro Shiota2, Masatoshi Maeki4, Akihiko Ishida4, Hirofumi Tani4, Koji Shigemura2, Akihide Hibara5, Manabu Tokeshi4
1Graduate School of Chemical Sciences and Engineering, Hokkaido University 1 , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
2Tianma Japan, Ltd. 2 , Shin-Kawasaki Mitsui Building West Tower 28F 1-1-2, Kashimada, Saiwai-ku, Kawasaki, Kanagawa 212-0058, Japan
3Department of Applied Science and Engineering, School of Engineering, Hokkaido University 3 , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
4Faculty of Engineering, Division of Applied Chemistry, Hokkaido University 4 , Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan
5Institute of Multidisciplinary Research for Advanced Materials, Tohoku University 5 , Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan

Tóm tắt

Fluorescence polarization (FP) offers easy operation and rapid processing, making it implementable in molecular interaction analysis. Previously we have developed a unique FP measurement system using a liquid crystal (LC) layer and an image sensor. The system is based on a principle of synchronized detection between the switching rate of the LC layer and the sampling rate of the CCD. The FP system realized simultaneous multiple sample detection; however, the measurement precision was lower than that of the conventional FP apparatus. The main drawbacks were low light transmittance of the LC layer and insufficient synchronization between the LC layer and CCD. In this paper, we developed a new FP analyzer based on LC-CCD synchronization detection. By using a newly designed LC with high transmittance and improving synchronization, the performance of the system has been dramatically improved. Additionally, we reduced the cost by using an inexpensive CCD and an LED as the excitation source. Simultaneous FP immunoassay of multiple samples of prostaglandin E2 was performed. The error rate of the FP system is reduced from 16.9% to 3.9%, as comparable to the commercial conventional FP system.

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Review of Scientific Instruments

Tập 89 Số 2

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