A chicken liver cell line efficiently supports the replication of ALV-J possibly through its high level viral receptor and efficient protein expression system
Tóm tắt
In this study, we identified a chicken liver cell line (LMH) which could strongly support the replication of ALV-J (Subgroup J of avian leukosis virus) with high viral titer. Notably, ALV-J was efficiently detected by ELISA in LMH cells 1 day before DF1 cells. In comparison with DF1 cells, LMH cells not only expressed higher levels of ALV-J receptor chNHE-1, but also possessed a more efficient protein expression system for foreign genes. Thus, LMH cells could be a novel tool to shorten the ALV-J eradication approach and accelerate studies on the pathogenesis and oncogenesis of ALV-J.
Tài liệu tham khảo
Payne LN, Brown SR, Bumstead N, Howes K, Frazier JA, Thouless ME (1991) A novel subgroup of exogenous avian leukosis virus in chickens. J Gen Virol 72:801–807
Payne LN, Howes K, Gillespie AM, Smith LM (1992) Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species: support for a new avian retrovirus envelope subgroup, designated J. J Gen Virol 73:2995–2997
Payne LN, Nair V (2012) The long view: 40 years of avian leukosis research. Avian Pathol 41:11–19
Maas R, van Zoelen D, Oei H, Claassen I (2006) Replacement of primary chicken embryonic fibroblasts (CEF) by the DF-1 cell line for detection of avian leucosis viruses. Biologicals 34:177–181
Yun B, Li D, Zhu H, Liu W, Qin L, Liu Z, Wu G, Wang Y, Qi X, Gao H, Wang X, Gao Y (2013) Development of an antigen-capture ELISA for the detection of avian leukosis virus p27 antigen. J Virol Methods 187:278–283
Qin A, Lee LF, Fadly A, Hunt H, Cui Z (2001) Development and characterization of monoclonal antibodies to subgroup J avian leukosis virus. Avian Dis 45:938–945
Qian K, Liang Y, Yin L, Shao H, Ye J, Qin A (2015) Development and evaluation of an immunochromatographic strip for rapid detection of capsid protein antigen p27 of avian leukosis virus. J Virol Methods 221:115–118
Yin L, Qin A, Qian K, Shao H, Jin W, Shi R, Liang Y, Hang B, Sun W (2013) Development of monoclonal antibodies against p27 of avian leukosis virus and the establishemnt of a sandwich ELISA for detection. China Poult 35:15–19
Chai N, Bates P (2006) Na+/H+ exchanger type 1 is a receptor for pathogenic subgroup J avian leukosis virus. Proc Natl Acad Sci U S A 103:5531–5536
Wang L, Mei M, Qin A, Ye J, Qian K, Shao H (2016) Membrane-associated GRP78 helps subgroup J avian leucosis virus enter cells. Vet Res 47:92
Gao Y, Qin L, Pan W, Wang Y, Le Q, Gao H, Wang X (2010) Avian leukosis virus subgroup J in layer chickens, China. Emerg Infect Dis 16:1637–1638
Cui N, Su S, Chen Z, Zhao X, Cui Z (2014) Genomic sequence analysis and biological characteristics of a rescued clone of avian leukosis virus strain JS11C1, isolated from indigenous chickens. J Gen Virol 95:2512–2522
Li X, Lin W, Chang S, Zhao P, Zhang X, Liu Y, Chen W, Li B, Shu D, Zhang H, Chen F, Xie Q (2016) Isolation, identification and evolution analysis of a novel subgroup of avian leukosis virus isolated from a local Chinese yellow broiler in South China. Arch Virol 161:2717–2725
Shao H, Wang L, Sang J, Li T, Liu Y, Wan Z, Qian K, Qin A, Ye J (2017) Novel avian leukosis viruses from domestic chicken breeds in mainland China. Arch Virol 162:2073–2076
Mei M, Ye J, Qin A, Wang L, Hu X, Qian K, Shao H (2015) Identification of novel viral receptors with cell line expressing viral receptor-binding protein. Sci Rep 5:7935
Chen B, Pan W, Zhang L, Liu J, Ouyang H, Nie Q, Zhang X (2014) NHE1 gene associated with avian leukosis virus subgroup J infection in chicken. Mol Biol Rep 41:6519–6524