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Khung tổ hợp hữu cơ kim loại bimetallic (Co/2Fe) với hoạt tính mô phỏng oxidase và peroxidase cho việc phát hiện hydrogen peroxide bằng phương pháp màu quang
Tóm tắt
Một khung tổ hợp hữu cơ kim loại bimetallic loại MOF(Co/2Fe), được tổng hợp bằng phương pháp thủy nhiệt, cho thấy khả năng hoạt động giống như peroxidase và oxidase. Vật liệu này xúc tác quá trình oxi hóa 3,3,5,5-tetramethylbenzidine (TMB) để tạo ra một sản phẩm màu xanh cả trong điều kiện không có và có mặt H2O2. Hoạt tính xúc tác của nó phụ thuộc mạnh mẽ vào nhiệt độ và giá trị pH của dung dịch. Với hoạt tính kép giống như enzyme, MOF(Co/2Fe) có những ưu điểm như chi phí thấp, độ ổn định tốt, dễ dàng trong việc chuẩn bị, và hành vi Michaelis-Menten hấp dẫn. Các cơ chế và động học của hoạt tính giống enzyme giả đã được nghiên cứu chi tiết. Một phương pháp màu quang đã được phát triển để xác định H2O2 dựa trên hoạt tính giống như peroxidase của MOF(Co/2Fe). Dưới các điều kiện tối ưu, độ hấp thụ của TMB đã được oxi hóa tại 652 nm tăng lên tuyến tính trong phạm vi nồng độ H2O2 từ 10 đến 100 µM, với giới hạn phát hiện là 5 µM.
Từ khóa
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