A Versatile Monosaccharide Transporter That Operates in the Arbuscular Mycorrhizal FungusGlomussp Is Crucial for the Symbiotic Relationship with Plants

Plant Cell - Tập 23 Số 10 - Trang 3812-3823 - 2011
Nicole Werner1, Kathrin Wippel2, Norbert Sauer2, Sara Schaarschmidt3, Bettina Hause3, Natalia Requena1
1Plant-Microbial Interactions Group, Karlsruhe Institute of Technology, Hertzstrasse 16, D-76187 Karlsruhe, Germany
2Friedrich-Alexander University Erlangen-Nürnberg, Molecular Plant-Physiology, D-91054 Erlangen, Germany
3Leibniz Institute of Plant Biochemistry, D-06018 Halle, Germany

Tóm tắt

AbstractFor more than 400 million years, plants have maintained a mutualistic symbiosis with arbuscular mycorrhizal (AM) fungi. This evolutionary success can be traced to the role of these fungi in providing plants with mineral nutrients, particularly phosphate. In return, photosynthates are given to the fungus, which support its obligate biotrophic lifestyle. Although the mechanisms involved in phosphate transfer have been extensively studied, less is known about the reciprocal transfer of carbon. Here, we present the high-affinity Monosaccharide Transporter2 (MST2) from Glomus sp with a broad substrate spectrum that functions at several symbiotic root locations. Plant cell wall sugars can efficiently outcompete the Glc uptake capacity of MST2, suggesting they can serve as alternative carbon sources. MST2 expression closely correlates with that of the mycorrhiza-specific Phosphate  Transporter4 (PT4). Furthermore, reduction of MST2 expression using host-induced gene silencing resulted in impaired mycorrhiza formation, malformed arbuscules, and reduced PT4 expression. These findings highlight the symbiotic role of MST2 and support the hypothesis that the exchange of carbon for phosphate is tightly linked. Unexpectedly, we found that the external mycelium of AM fungi is able to take up sugars in a proton-dependent manner. These results imply that the sugar uptake system operating in this symbiosis is more complex than previously anticipated.

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Plant Cell

Tập 23 Số 10

3812-3823

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