A Protein Sequenator

FEBS Journal - Tập 1 Số 1 - Trang 80-91 - 1967
Pehr Edman1, Geoffrey S. Begg1
1St. Vincent's School of Medical Research Melbourne, N. 6, Victoria, Australia

Tóm tắt

The protein sequenator is an instrument for the automatic determination of amino acid sequences in proteins and peptides. It operates on the principle of the phenylisothiocyanate degradation scheme. The automated process embraces the formation of the phenylthiocarbamyl derivative of the protein and the splitting off of the N‐terminal amino acid as thiazolinone. The degradation proceeds at a rate of 15.4 cycles in 24 hours and with a yield in the individual cycle in excess of 98%. The material requirements are approximately 0.25 μmoles of protein. The thiazolinones are converted to the corresponding phenylthiohydantoins in a separate operation, and the latter identified by thin layer chromatography. The process has been applied to the whole molecule of apomyoglobin from the humpback whale, and it has been possible to establish the sequence of the first 60 amino acids from the N‐terminal end.

Từ khóa


Tài liệu tham khảo

10.3891/acta.chem.scand.10-0761

Bethell D., 1965, Chem. Comm., 10, 189

10.1071/CH9630411

Edman P., 1963, Thromb. et Diath. Haemorrhag., 17

Fischer A., 1954, Z. Naturforsch., 9, 508, 10.1515/zna-1954-0604

10.1016/0304-4165(66)90437-5

10.1039/jr9560000188

Edman P., 1957, Proc. Roy. Australian Chem. Inst., 434

Teremoto S., 1954, Hakko Kogaku Zasshi, 32, 350

10.1098/rspa.1957.0002

10.3891/acta.chem.scand.13-1542

Theorell H., 1954, Ann. Acad. Sci. Fennicae Ser. A II, 60, 303

10.3891/acta.chem.scand.10-1507

10.1002/hlca.19610440123

10.1038/205883a0

10.1021/jo01094a627

10.1021/bi00887a031

Thông tin
Thông tin xuất bản

FEBS Journal

Tập 1 Số 1

80-91

Thông tin tác giả