A Persistent Activity-Dependent Facilitation in Chromaffin Cells Is Caused by Ca2+Activation of Protein Kinase C

Journal of Neuroscience - Tập 19 Số 2 - Trang 589-598 - 1999
Corey L. Smith1
1Abteilung Neurobiologie, MPI for biophysical chemistry, Max Planck Society

Tóm tắt

Activity-dependent facilitation was studied in bovine adrenal chromaffin cells. Stimulation with a train of depolarizations caused subsequent triggered exocytotic activity to be significantly enhanced. After the facilitating stimulus train, the readily releasable vesicle pool (RRP) size was estimated from capacitance jumps in response to paired depolarizations and found to be elevated for a period of at least 10 min. The time dependency of onset and degree of facilitation could be well fitted assuming protein kinase C (PKC)-dependent and independent Ca2+-mediated processes. Both processes increase the recruitment of vesicles from the reserve pool to the RRP, resulting in an greater number of releasable vesicles. The data suggest that cell activity can act as a trigger to increase cytosolic Ca2+to a level sufficient to cause an increase in the number of readily releasable secretory vesicles, with the more persistent component of the evoked facilitation being mediated through activity-dependent activation of PKC.

Từ khóa


Tài liệu tham khảo

10.1038/363356a0

10.1073/pnas.91.10.4343

10.1113/jphysiol.1970.sp009034

Bittner, 1992, Kinetic analysis of secretion from permeabilized adrenal chromaffin cells reveals distinct components., J Biol Chem, 267, 16219, 10.1016/S0021-9258(18)41988-6

10.1007/BF00374652

10.1523/JNEUROSCI.16-02-00425.1996

10.1073/pnas.91.26.12765

10.1016/S0896-6273(00)80054-9

10.1016/S0896-6273(00)80126-9

10.1113/jphysiol.1965.sp007639

10.1523/JNEUROSCI.16-04-01359.1996

10.1111/j.1469-7793.1998.591bv.x

10.1523/JNEUROSCI.17-23-09010.1997

10.1016/S0166-2236(96)01001-6

10.1016/S0896-6273(00)81017-X

Gillis KD (1995) Chapter 7. Techniques for membrane capacitance measurements. in Single-channel recording, Ed 2, eds Sakmann B Neher E (Plenum, New York), pp 155–198.

10.1016/S0896-6273(00)80147-6

10.1126/science.8430330

Grynkiewicz, 1985, A new generation of Ca2+ indicators with greatly improved fluorescence properties., J Biol Chem, 260, 3440, 10.1016/S0021-9258(19)83641-4

10.1007/BF00374600

10.1016/S0006-3495(94)80744-1

10.1016/0896-6273(94)90050-7

10.1016/S0896-6273(00)80182-8

10.1016/S0006-3495(97)78704-6

Knight, 1983, Stimulus-secretion coupling in isolated bovine adrenal medullary cells., Q J Exp Physiol, 68, 123, 10.1113/expphysiol.1983.sp002691

Kumar, 1998, Release of dopamine and norepinephrine by hypoxia from PC-12 cells., Am J Physiol, 274, C1592, 10.1152/ajpcell.1998.274.6.C1592

10.1073/pnas.92.20.9235

10.1007/BF00582306

10.1523/JNEUROSCI.07-05-01567.1987

10.1111/j.1469-7793.1998.605bk.x

10.1016/0092-8674(94)90517-7

10.1523/JNEUROSCI.17-07-02314.1997

10.1016/S0896-6273(00)80983-6

10.1113/jphysiol.1992.sp019127

10.1073/pnas.79.21.6712

10.1016/0896-6273(93)90238-M

10.1111/j.1469-7793.1997.105bl.x

10.1016/S0034-5687(97)00077-7

10.1523/JNEUROSCI.16-02-00553.1996

10.1083/jcb.139.4.885

10.1016/S0896-6273(00)80504-8

10.1073/pnas.92.3.846

10.1083/jcb.138.1.55

10.1016/S0896-6273(00)81248-9

TerBush, 1988, Ca2+ influx causes rapid translocation of protein kinase C to membranes. Studies of the effects of secretagogues in adrenal chromaffin cells., J Biol Chem, 263, 18873, 10.1016/S0021-9258(18)37363-0

10.1111/j.1469-7793.1997.705bd.x

10.1016/0896-6273(93)90274-U

10.1016/0896-6273(95)90291-0

10.1083/jcb.134.2.329

10.1126/science.8235626

10.1016/S0896-6273(00)80523-1

10.1038/642

10.1016/S0006-3495(97)78091-3

10.1016/S0896-6273(00)80341-4

10.1074/jbc.270.8.3498

10.1113/jphysiol.1993.sp019813

10.1146/annurev.ne.12.030189.000305

10.1016/S0896-6273(00)80238-X

Thông tin
Thông tin xuất bản

Journal of Neuroscience

Tập 19 Số 2

589-598

Thông tin tác giả