A Novel Water-soluble Ratiometric Fluorescent Probe Based on FRET for Sensing Lysosomal pH

Journal of Fluorescence - Tập 26 - Trang 2079-2086 - 2016
Guang-Jie Song1,2, Su-Yun Bai3,4, Jing Luo1, Xiao-Qun Cao1, Bao-Xiang Zhao2
1School of Chemical Engineering, Taishan Medical University, Tai’an, China
2Institute of Organic Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Jinan, China
3Institute of Developmental Biology, School of Life Science, Shandong University, Jinan, China
4School of Life Science, Taishan Medical University, Tai’an, China

Tóm tắt

A new ratiometric fluorescent probe based on Förster resonance energy transfer (FRET) for sensing lysosomal pH has been developed. The probe (RMPM) was composed of imidazo[1,5-α]pyridine quaternary ammonium salt fluorophore as the FRET donor and the rhodamine moiety as the FRET acceptor. It’s the first time to report that imidazo[1,5-α]pyridine quaternary ammonium salt acts as the FRET donor. The ratio of fluorescence intensity of the probe at two wavelengths (I424/I581) changed significantly and responded linearly toward minor pH changes in the range of 5.4–6.6. It should be noted that it’s rare to report that a ratiometric pH probe could detect so weak acidic pH with pKa = 6.31. In addition, probe RMPM exhibited excellent water-solubility, fast-response, all-right selectivity and brilliant reversibility. Moreover, RMPM has been successfully applied to sensing lysosomal pH in HeLa cells and has low cytotoxicity.

Tài liệu tham khảo

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