John F. Kearney1,2, Andreas Radbruch1,2, B Liesegang1,2, Klaus Rajewsky1,2
1Cellular Immunobiology Unit, University of Alabama in Birmingham , Alabama 35294 , and the , Weyertal 121, D-500 Köln 41, F. R. G.
2Institute for Genetics, University of Cologne , Alabama 35294 , and the , Weyertal 121, D-500 Köln 41, F. R. G.
Tóm tắt
Abstract
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.
