A KINETIC ISOTOPE EFFECT IN THE PHOTOREACTIVATION OF U.V.‐INACTIVATED TOBACCO MOSAIC VIRUS RNA*

Photochemistry and Photobiology - Tập 9 Số 3 - Trang 243-246 - 1969
N. A. Evans1, Angus McLaren1
1College of Agricultural Sciences, University of California, Berkeley. Calif. 94720, U.S.A.

Tóm tắt

Abstract— Inactivation of tobacco mosaic virus RNA (TMV‐RNA) by u.v. radiation is slower in D2O than in H2O, and TMV‐RNA which has been inactivated in D2O is photoreactivated faster (on Pinto bean) than TMV‐RNA which has been inactivated in H2O. The maximum amount of photoreactivation is unaffected by the solvent, H2O or D2O, present during irradiation. These deuterium isotope effects for inactivation and photoreactivation suggest that pyrimidine hydrates are photoreactivable lesions on Pinto bean.

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Tài liệu tham khảo

10.1038/183503a0

Schuster H., 1964, Z. Naturforsch., 19, 815, 10.1515/znb-1964-0909

10.1111/j.1751-1097.1967.tb08879.x

G. D.Small.M.TaoandM. P.Gordon J. Mol. Biol.in press.

10.1021/bi00876a051

Knight C. A., 1962, Biochem. Preparations, 9, 132

Fraenkel‐Conrat H., 1966, Procedures in Nucleic Acid Research, 480

10.2307/3570450

Evens N. A., 1968, J. Gen. Virology, 4, 333

10.1016/0042-6822(66)90143-7

O.Hidalgo‐SalvatierraandA. D.McLaren Phorochem. Photobiol.To be published (1969).

10.1021/ja01010a055

10.1002/9780470171806.ch4