A DiCre recombinase-based system for inducible expression in Leishmania major

Molecular and Biochemical Parasitology - Tập 216 - Trang 45-48 - 2017
Renato Elias Rodrigues de Souza Santos1,2, Gabriel L A Silva1,2, Elaine Vieira Santos1,2, Samuel M. Duncan3, Jeremy C. Mottram4,3, Jeziel D. Damasceno1,2, Luíz Ricardo Orsini Tosi1,2
1Department of Cell and Molecular Biology, Ribeirão Preto Medical School, University of São Paulo
2Ribeirão Preto, SP, Brazil
3Wellcome Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, United Kingdom
4Centre for Immunology and Infection, Department of Biology, University of York, York, United Kingdom

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Tài liệu tham khảo

Alvar, 2012, Leishmaniasis worldwide and global estimates of its incidence, PLoS One, 7, e35671, 10.1371/journal.pone.0035671

Martı́nez-Calvillo, 2003, Transcription of Leishmania major friedlin chromosome 1 initiates in both directions within a single region, Mol. Cell., 11, 1291, 10.1016/S1097-2765(03)00143-6

Ubeda, 2008, Modulation of gene expression in drug resistant Leishmania is associated with gene amplification, gene deletion and chromosome aneuploidy, Genome Biol., 9, R115, 10.1186/gb-2008-9-7-r115

Rogers, 2011, Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania, Genome Res., 21, 2129, 10.1101/gr.122945.111

LeBowitz, 1991, Simultaneous transient expression assays of the trypanosomatid parasite Leishmania using β-galactosidase and β-glucuronidase as reporter enzymes, Gene, 103, 119, 10.1016/0378-1119(91)90402-W

Damasceno, 2010, A transposon toolkit for gene transfer and mutagenesis in protozoan parasites, Genetica, 138, 301, 10.1007/s10709-009-9406-7

Duncan, 2017, Recent advances in Leishmania reverse genetics: Manipulating a manipulative parasite, Mol. Biochem. Parasitol., 216, 30, 10.1016/j.molbiopara.2017.06.005

Madeira da Silva, 2009, Regulated expression of the Leishmania major surface virulence factor lipophosphoglycan using conditionally destabilized fusion proteins, PNAS, 106, 7583, 10.1073/pnas.0901698106

Podešvová, 2017, Inducible protein stabilization system in Leishmania mexicana, Mol. Biochem. Parasitol., 214, 62, 10.1016/j.molbiopara.2017.03.008

Kraeva, 2014, Tetracycline-inducible gene expression system in Leishmania mexicana, Mol. Biochem. Parasitol., 198, 11, 10.1016/j.molbiopara.2014.11.002

Duncan, 2016, Conditional gene deletion with DiCre demonstrates an essential role for CRK3 in Leishmania mexicana cell cycle regulation, Mol. Microbiol., 100, 931, 10.1111/mmi.13375

Beneke, 2017, A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids, R. Soc. Open Sci., 4, 170095, 10.1098/rsos.170095

Ran, 2013, Genome engineering using the CRISPR-Cas9 system, Nat. Protoc., 8, 2281, 10.1038/nprot.2013.143

Van Duyne, 2001, A structural view of cre- loxP site-Specific recombination, Annu. Rev. Biophys. Biomol. Struct., 30, 87, 10.1146/annurev.biophys.30.1.87

Jullien, 2003, Regulation of Cre recombinase by ligand-induced complementation of inactive fragments, Nucleic Acids Res., 31, e131, 10.1093/nar/gng131

Albert, 1995, Site-specific integration of DNA into wild-type and mutant lox sites placed in the plant genome, Plant J., 7, 649, 10.1046/j.1365-313X.1995.7040649.x

Damasceno, 2013, LmHus1 is required for the DNA damage response in Leishmania major and forms a complex with an unusual Rad9 homologue, Mol. Microbiol., 90, 1074, 10.1111/mmi.12418

Damasceno, 2016, Functional compartmentalization of Rad9 and Hus1 reveals diverse assembly of the 9-1-1 complex components during the DNA damage response in Leishmania, Mol. Microbiol., 101, 1054, 10.1111/mmi.13441

Navadgi-Patil, 2009, The unstructured C-terminal tail of the 9-1-1 clamp subunit Ddc1 activates Mec1/ATR via two distinct mechanisms, Mol. Cell., 36, 743, 10.1016/j.molcel.2009.10.014