A 38-Kilobase Pathogenicity Island Specific for<i>Mycobacterium avium</i>subsp.<i>paratuberculosis</i>Encodes Cell Surface Proteins Expressed in the Host

Infection and Immunity - Tập 72 Số 3 - Trang 1265-1274 - 2004
Janin Stratmann1, Birgit Strommenger1, Ralph Goethe1, Karen Dohmann1, Gerald‐F. Gerlach1, Karen Stevenson2, Yannick Pauchet3, Qing Zhang3, Vivek Kapur3, Tim J. Bull4
1Institute for Microbiology, Department of Infectious Diseases, School of Veterinary Medicine, Hannover, Germany
2Moredun Research Institute, International Research Centre, Penicuik, Scotland
3Department of Microbiology and Biomedical Genomics Center, University of Minnesota, Minneapolis, Minnesota
4Department of Surgery, St. George's Hospital Medical School, London, United Kingdom

Tóm tắt

ABSTRACTWe have used representational difference analysis to identify a novelMycobacterium aviumsubsp.paratuberculosis-specific ABC transporter operon (mpt), which comprises six open reading frames designatedmptAto -Fand is immediately preceded by two putative Fur boxes. Functional genomics revealed that themptoperon is flanked on one end by afepcluster encoding proteins involved in the uptake of Fe3+and on the other end by asidcluster encoding non-ribosome-dependent heterocyclic siderophore synthases. Together these genes form a 38-kbM. aviumsubsp.paratuberculosis-specific locus flanked by an insertion sequence similar to IS1110. Expression studies using Western blot analyses showed that MptC is present in the envelope fraction ofM. aviumsubsp.paratuberculosis. The MptD protein was shown to be surface exposed, using a specific phage (fMptD) isolated from a phage-peptide library, by differential screening ofMycobacterium smegmatistransformants. The phage fMptD-derived peptide could be used in a peptide-mediated capture PCR with milk from infected dairy herds, thereby showing surface-exposed expression of the MptD protein in the host. Together, these data suggest that the 38-kb locus constitutes anM. aviumsubsp.paratuberculosispathogenicity island.

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Tài liệu tham khảo

10.1128/JB.184.21.5826-5832.2002

10.1016/S0378-1119(96)00650-6

10.1128/iai.65.5.1659-1668.1997

10.1038/417141a

10.1023/A:1022443209010

10.1093/nar/21.10.2529

10.1046/j.1365-2958.2001.02414.x

10.1128/IAI.69.11.6702-6706.2001

Burlein, J. E., C. K. Stover, S. Offutt, and M. S. Hanson. 1994. Expression of foreign genes in mycobacteria, p. 239-252. In B. R. Bloom (ed.), Tuberculosis: pathogenesis, protection, and control. ASM Press, Washington, D.C.

10.1099/13500872-140-8-1977

10.1016/S1286-4579(01)01412-5

10.1128/CMR.2.1.90

Chiodini, R. J., H. J. Van Kruiningen, and R. S. Merkal. 1984. Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet.74:218-262.

Cole, S. T., R. Brosch, J. Parkhill, T. Garnier, C. Churcher, D. Harris, S. V. Gordon, K. Eiglmeier, S. Gas, C. E. Barry, F. Tekaia, K. Badcock, D. Basham, D. Brown, T. Chillingworth, R. Connor, R. Davies, K. Devlin, T. Feltwell, S. Gentles, N. Hamlin, S. Holroyd, T. Hornby, K. Jagels, A. Krogh, J. McLean, S. Moule, L. Murphy, K. Oliver, J. Osborne, M. A. Quail, M. A. Rajandream, J. Rogers, S. Rutter, K. Seeger, J. Skelton, R. Squares, S. Squares, J. E. Sulston, K. Taylor, S. Whitehead, and B. G. Barrell. 1998. Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature396:190-198.

10.1002/j.1460-2075.1993.tb06038.x

10.1128/jcm.35.7.1794-1799.1997

10.1128/mr.57.4.995-1017.1993

10.1046/j.1365-2958.1999.01348.x

10.1128/iai.60.3.892-898.1992

10.1016/S1286-4579(01)01411-3

10.1128/CMR.14.3.489-512.2001

10.1155/2000/798305

10.1111/j.1365-2958.1994.tb01059.x

10.1146/annurev.cb.08.110192.000435

10.1128/IAI.66.2.710-716.1998

10.1111/j.1572-0241.2001.03751.x

Kertesz, M. A. 2000. Riding the sulfur cycle—metabolism of sulfonates and sulfate esters in gram-negative bacteria. FEMS Microbiol. Rev.24:135-175.

10.1177/104063879100300425

10.1126/science.8438152

10.1016/S0168-9525(00)89087-3

10.1093/infdis/155.5.1011

10.1128/iai.65.2.387-394.1997

Mutwiri, G. K., U. Kosecka, M. Benjamin, S. Rosendal, M. Perdue, and D. G. Butler. 2001. Mycobacterium avium subspecies paratuberculosis triggers intestinal pathophysiologic changes in beige/scid mice. Comp. Med.51:538-544.

10.1111/j.1574-6968.2002.tb11347.x

Current protocols in molecular biology 1989

10.1046/j.1365-2958.2003.03384.x

Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.

Sander, P., and E. C. Boettger. 1998. Gene replacement in Mycobacterium smegmatis using a dominant negative selectable marker, p. 207-216. In T. Parish and N. G. Stoker (ed.), Mycobacteria protocols. Humana Press Inc., Totowa, N.J.

10.1073/pnas.212527599

10.1128/JB.181.20.6387-6395.1999

10.1128/IAI.69.4.2075-2082.2001

10.1111/j.1365-2958.1990.tb02040.x

Snyder L. and W. Champness. 1997. Molecular genetics of bacteria p. 55. ASM Press Washington D.C.

10.1128/JCM.40.11.4244-4250.2002

10.1111/j.1574-6968.2001.tb10536.x

Tanghe, A., P. Lefevre, O. Denis, S. D'Souza, M. Braibant, E. Lozes, M. Singh, D. Montgomery, J. Content, and K. Huygen. 1999. Immunogenicity and protective efficacy of tuberculosis DNA vaccines encoding putative phosphate transport receptors. J. Immunol.162:1113-1119.

10.1099/00221287-144-12-3413

10.1128/IAI.67.4.1974-1981.1999

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Infection and Immunity

Tập 72 Số 3

1265-1274

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