A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: Application for DNA fragment transfer between chromosomes and plasmid transformation

Journal of Microbiological Methods - Tập 64 Số 3 - Trang 391-397 - 2006
Kyoung‐Hee Choi1, Ayush Kumar2, Herbert P. Schweizer2
1Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, USA
2Department of Microbiology, Immunology, and Pathology, Colorado State University, 1682 Campus Delivery, Fort Collins, CO 80523, United States.

Tóm tắt

Từ khóa


Tài liệu tham khảo

Bao, 1991, An improved Tn7-based system for the single-copy insertion of cloned genes into the chromosomes of Gram-negative bacteria, Gene, 109, 167, 10.1016/0378-1119(91)90604-A

Budzik, 2004, Isolation and characterization of a generalized transducing phage for Pseudomonas aeruginosa strains PAO1 and PA14, J. Bacteriol., 186, 3270, 10.1128/JB.186.10.3270-3273.2004

Choi, 2005, A Tn7-based broad-range bacterial cloning and expression system, Nature Methods, 2, 443, 10.1038/nmeth765

Darzins, 1989, Mini-D3112 bacteriophage transposable elements for genetic analysis of Pseudomonas aeruginosa, J. Bacteriol., 171, 3909, 10.1128/jb.171.7.3909-3916.1989

Dennis, 1995, Electrotransformation of Pseudomonas, 125

Diver, 1990, Transformation of Pseudomonas aeruginosa by electroporation, Anal. Biochem., 189, 75, 10.1016/0003-2697(90)90046-C

Enderle, 1998, Electroporation of freshly plated Escherichia coli and Pseudomonas aeruginosa cells, BioTechniques, 25, 954, 10.2144/98256bm05

Farinha, 1990, High efficiency electroporation of Pseudomonas aeruginosa using frozen cell suspensions, FEMS Microbiol. Lett., 58, 221

Gallagher, 2002, Functions required for extracellular quinolone signaling by Pseudomonas aeruginosa, J. Bacteriol., 184, 6472, 10.1128/JB.184.23.6472-6480.2002

Hoang, 1998, A broad-host-range Flp–FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants, Gene, 212, 77, 10.1016/S0378-1119(98)00130-9

Hoang, 2000, Integration proficient plasmids for Pseudomonas aeruginosa: site-specific integration and use for engineering of reporter and expression strains, Plasmid, 43, 59, 10.1006/plas.1999.1441

Holloway, 1955, Genetic recombination in Pseudomonas aeruginosa, J. Gen. Microbiol., 13, 572, 10.1099/00221287-13-3-572

Holloway, 1959, Transduction in Pseudomonas aeruginosa, Nature, 184, 1326, 10.1038/1841426b0

Irani, 1997, Enhancement of transformation in Pseudomonas aeruginosa PAO1 by Mg2+ and heat, BioTechniques, 22, 54, 10.2144/97221bm09

Ishimoto, 1989, Formation of pilin in Pseudomonas aeruginosa requires the alternative sigma factor (RpoN) of RNA polymerase, Proc. Natl. Acad. Sci. U. S. A., 86, 1954, 10.1073/pnas.86.6.1954

Jacobs, 2003, Comprehensive transposon mutant library of Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. U. S. A., 100, 14339, 10.1073/pnas.2036282100

Krishnapillai, 1971, A novel transducing phage, Mol. Gen. Genet., 114, 134, 10.1007/BF00332784

Kulasekara, 2005, A novel two-component system controls the expression of Pseudomonas aeruginosa fimbrial cup genes, Mol. Microbiol., 55, 368, 10.1111/j.1365-2958.2004.04402.x

Morgan, 1979, Transduction of Pseudomonas aeruginosa with a mutant of phage E79, J. Bacteriol., 139, 137, 10.1128/JB.139.1.137-140.1979

Peters, 2001, Tn7: smarter than we thought, Nat. Rev., Mol. Cell Biol., 2, 806, 10.1038/35099006

Quenee, 2005, Combined sacB-based negative selection and cre-lox antibiotic marker recycling for efficient gene deletion in Pseudomonas aeruginosa, BioTechniques, 38, 63, 10.2144/05381ST01

Rahme, 1995, Common virulence factors for bacterial pathogenicity in plants and animals, Science, 268, 1899, 10.1126/science.7604262

Sambrook, 2001

Smith, 1989, Transformation of Pseudomonas aeruginosa by electroporation, Nucl. Acids Res., 17, 10509, 10.1093/nar/17.24.10509

Stover, 2000, Complete genome sequence of Pseudomonas aeruginosa, an opportunistic pathogen, Nature, 406, 959, 10.1038/35023079

West, 1994, Construction of improved Escherichia–Pseudomonas shuttle vectors derived from pUC18/19 and the sequence of the region required for their replication in Pseudomonas aeruginosa, Gene, 128, 81, 10.1016/0378-1119(94)90237-2

Wong, 2000, Genetic footprinting with mariner-based transposition in Pseudomonas aeruginosa, Proc. Natl. Acad. Sci. U. S. A., 97, 10191, 10.1073/pnas.97.18.10191