In vitro interactions between thymocytes and hemopoietic precursor cells
Tóm tắt
The effects of thymocytes on in vitro hemopoiesis were evaluated by co-culturing mixtures of mouse marrow cells and syngeneic thymocytes for granulocyte/macrophage precursors (CFU-GM), and for early (BFU-E) and late (CFU-E) erythroid precursors. Co-cultures were studied which contained fixed numbers of marrow cells with increasing numbers of thymocytes as well as co-cultures in which the total number of cells was kept constant. In addition, the effects of thymocytes on cultures stimulated by optimal or suboptimal amounts of humoral factors, the effects of a liquid phase incubation of the marrow cells with thymocytes prior to semi-solid culture, and the effects of irradiated and disrupted thymocytes were studied. The growth of CFU-GM was stimulated by thymocytes less than the growth of the other progenitor cells studied; at most, minimal stimulation by thymocytes was detected and at some marrow cell: thymocyte (M:T) ratios inhibition was even suggested. The growth of BFU-E was more strongly and more consistently stimulated than that of CFU-E. Killed thymocytes did not effect stimulation of CFU-E or BFU-E. These studies suggest that, in unfractionated marrow cells, the various hemopoietic precursor cells differ in their sensitivities to in vitro stimulation by thymocytes. In addition, it appears that only viable thymocytes can effect stimulation of the hemopoietic precursors in the vitro culture systems used.
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