Putative lmbI and lmbH genes form a single lmbIH ORF in Streptomyces lincolnensis type strain ATCC 25466

Antonie van Leeuwenhoek - 2001
Jiøí Janata1, Lucie Najmanová1, Jitka Novotná1, Klára Holá1, Jürgen Felsberg1, Jaroslav Spížek1
1Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague 4, Czech Republic

Tóm tắt

The lincomycin-production gene cluster of the industrial overproduction strain Streptomyces lincolnensis 78-11 has been sequenced (Peschke et al. 1995) and twenty-seven putative open reading frames with biosynthetic or regulatory functions (lmb genes) identified. Two distinct hypothetical genes, lmbI and lmbH, were found downstream of the lmbJ gene, coding for LmbJ protein, which is believed to participate in the last lincomycin biosynthetic step, i.e. conversion of N-demethyllincomycin (NDL) to lincomycin. In the present study, we demonstrate the presence of a single larger open reading frame, called lmbIH, in the lincomycin low-production type strain Streptomyces lincolnensis ATCC 25466, instead of two smaller lmbI and lmbH genes. The product, LmbIH, is a protein of an unknown function and is homologous with the TldD protein family. Escherichia coli TldD protein was previously shown to be involved in the control of DNA gyrase by LetD protein. Moreover, our experiments indicate co-regulation of lmbJ and lmbIH expression. This translation coupling probably reflects an eight nucleotide overlap between the lmbJ and lmbIH genes, as well as the lack of a Shine-Dalgarno sequence upstream of the lmbIH gene.

Từ khóa


Tài liệu tham khảo

Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W & Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 25: 3389-3402

Ausubel FA, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA & Struhl K (1998) Current protocols in molecular biology. John Wiley & Sons, New York

Chung S-TC, Manis JJ, McWethy SJ, Patt TE, Witz DF, Wolf HJ & Wovcha MG (1997) Fermentation, biosynthesis, and molecular genetics of lincomycin. In: Strohl WR (Ed) Biotechnology of industrial antibiotics (pp 165-186). Dekker, New York

Heilig JS, Elbing KL & Brent R (1998) Large-scale preparation of plasmid DNA. In: Ausubel FA, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA & Struhl K (Ed) Current protocols in molecular biology (pp 1.7.1-1.7.16). John Wiley & Sons, New York

Higgins DG & Sharp PM (1989) Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5: 151-153

Hopwood DA, Bibb MJ, Chater KF, Kieser T, Bruton CJ, Kieser HM, Lydiate DJ, Smith CP, Ward JM & Schrempf H (1985) Genetic manipulation of Streptomyces. A Laboratory Manual. The John Innes Foundation, Norwich, UK

Liu T, Zhang H, Chen Y & Ding J (1997) Retransformation of lincomycin biosynthesis gene lmbH. 10th International Symposium on Biology of Actinomycetes, abstract: 1P28. May 23-30, Beijing, China

Murayama N, Shimizu H, Takiguchi S, Baba Y, Amino H, Horiuchi T, Sekimizu K & Miki T (1996) Evidence for involvement of Escherichia coli genes pmbA, csrA and a previously unrecognized gene tldD, in the control of DNA gyrase by letD (ccdB) of sex factor F. J. Mol. Biol. 256: 483-502

Murray V (1989) Improved double-stranded DNA sequencing using linear polymerase chain reaction. Nucleic Acids Res. 17: 8889

Neusser D, Schmidt H, Spížek J, Novotná J, Peschke U, Kaschabeck S, Tichý P & Piepersberg W(1998) The genes lmbB1 and lmbB2 of Streptomyces lincolnensis encode enzymes involved in the conversion of L-tyrosine to propylproline during the biosynthesis of the antibiotic lincomycin A. Arch. Microbiol. 169: 322-332

Nishimura A, Morita M, Nishimura Y & Sugino Y (1990) A rapid and highly efficient method for preparation of competent Escherichia coli cells. Nucleic Acids Res. 18: 6169

Peschke U, Schmidt H, Zhang H-Z & Piepersberg W (1995) Molecular characterization of the lincomycin-production gene cluster of Streptomyces lincolnensis 78-11. Mol. Microbiol. 16: 1137-1156

Sanger F, Nicklen S & Coulson AR (1977) DNA sequencing with chain-terminator inhibitors. Proc. Nat. Acad. Sci. (USA) 74: 5463-5467

Thông tin
Thông tin xuất bản

Antonie van Leeuwenhoek

Thông tin tác giả