Sweet potato (Ipomoea batatas) trypsin inhibitors expressed in transgenic tobacco plants confer resistance against Spodoptera litura

Plant Cell Reports - Tập 16 - Trang 696-699 - 1997
K.-W. Yeh1, M.-I. Lin1, S.-J. Tuan2, Y.-M. Chen1, C.-J. Lin1, S.-S. Kao2
1Department of Botany, National Taiwan University, Taipei, 106, Taiwan, ROC, Fax no.: +886-2-3622703, E-mail: [email protected], , TW
2Department of Biopesticide, Taiwan Agricultural Chemicals and Toxic Substance Research Institute, Taichung Hsien, Taiwan, ROC, E-mail: [email protected], , TW

Tóm tắt

A sweet potato (Ipomoea batatas cv. Tainong 57) trypsin inhibitor gene was introduced into tobacco plants (Nicotiana tabaccum cv. W38) by Agrobacterium tumefaciens– mediated transformation. From 30 independent transformants, three lines with high level of expression were further analyzed. The trypsin inhibitor gene, under control of the 35S CaMV promoter, led to the production of the trypsin inhibitor proteins up to 0.2% of the total protein. In insecticidal bioassays of transgenic tobacco plants, larval, growth of Spodoptera litura (F.), the tobacco cutworm, was severely retarded as compared to their growth on control plants. This observation implied that expression of sweet potato trypsin inhibitor can provide an efficient method for crop protection.