The Drosophila melanogaster condensin subunit Cap-G interacts with the centromere-specific histone H3 variant CID

Chromosoma - Tập 113 - Trang 350-361 - 2004
Hubert Jäger1,2, Melanie Rauch1,3, Stefan Heidmann1
1Lehrstuhl für Genetik, University of Bayreuth, Bayreuth, Germany
2Astra-Zeneca Ltd, Wedel, Germany
3Department for Clinical and Biological Sciences and Department of Molecular Immunology Pharmacenter, University of Basel, Basel, Switzerland

Tóm tắt

The centromere-specific histone H3 variant CENP-A plays a crucial role in kinetochore specification and assembly. We chose a genetic approach to identify interactors of the Drosophila CENP-A homolog CID. Overexpression of cid in the proliferating eye imaginal disk results in a rough eye phenotype, which is dependent on the ability of the overexpressed protein to localize to the kinetochore. A screen for modifiers of the rough eye phenotype identified mutations in the Drosophila condensin subunit gene Cap-G as interactors. Yeast two-hybrid experiments also reveal an interaction between CID and Cap-G. While chromosome condensation in Cap-G mutant embryos appears largely unaffected, massive defects in sister chromatid segregation occur during mitosis. Taken together, our results suggest a link between the chromatin condensation machinery and kinetochore structure.

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