Induction of NADPH-quinone reductase as a screening assay for potential chemopreventive agents
Tóm tắt
An in vitro biochemical assay using induction of a phase II enzyme, NADPH-quinone reductase (NADPH-QR) for selection and identification of potential chemopreventive agents is described. A normal human liver cell line (Chang liver cells) or a rodent rat liver cell line (buffalo rat liver cells) was selected as the candidate cell line for induction of QR by a known chemopreventive agent, genistein. The choice of a liver cell line is based on the fact that liver being a major metabolic organ, phase II enzymes are commonly elaborated in liver tissues and is therefore appropriate for enzyme induction studies.
Tài liệu tham khảo
Prochaska HJ, Fernandes CL (1993). Elevation of serum phase II enzymes by anticarcinogenic enzyme inducers: Markers for a chemoprotected state? Carcinogenesis 14: 2441–2445.
Talalay P, Prochaska HJ (1987). In: Ernster L, Estabrook RW, Hochstein P, Orrenius S (eds), DT diaphorase: A quinone reductase with special functions in cell metabolism and detoxication. Cambridge, UK: Cambridge University Press, pp 62–66.
Talalay P, Benson AM (1982). Elevation of quinone reductase activity by anticarcinogenic antioxidants. Advan Enzyme Regul 20: 287–300.
Prochaska HJ, Santamaria AB (1988). Direct measurement of NAD(P)H: Quinone redutase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers. Anal Biochem 169: 328–336.