Molecular imprinting resonance light scattering nanoprobes based on pH-responsive metal-organic framework for determination of hepatitis A virus

Microchimica Acta - Tập 187 - Trang 1-8 - 2020
Lianghui Luo1, Feng Zhang2, Chunyan Chen1, Changqun Cai1
1Key Laboratory for Green Organic Synthesis and Application of Hunan Province, Key Laboratory of Environmentally Friendly Chemistry and Application of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, China
2School of Chemistry and Materials Science, Hunan Agricultural University, Changsha, China

Tóm tắt

Molecularly imprinted polymer (HM@MIP) nanoprobes were designed form the pH-responsive polymer (dimethylaminoethyl methacrylate (DMA)) and MIL-101. This probe was applied to the selective determination of hepatitis A virus (HAV) through Resonance light scattering (RLS) technique. DMA adjusts pH of the system to facilitate the capture and release of virus by HM@MIPs as anticipated. And it results in the enhancement or weaken of RLS intensity. According to RLS intensity at 470 nm, a linear concentration of 0.02–2.0 nmol·L−1 and a limit of detection of 0.1 pmol·L−1 were obtained within 20 min. The excellent recoveries ranges from 88% to 107%, and it indicates the prominent ability of the HM@MIPs to determination HAV in human serum and their potential ability to determination virus in real applications.

Tài liệu tham khảo

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