Dengue virus replicons: Production of an interserotypic chimera and cell lines from different species, and establishment of a cell-based fluorescent assay to screen inhibitors, validated by the evaluation of ribavirin's activity

Alvarez, 2008, Functional analysis of dengue virus cyclization sequences located at the 5’ and 3’UTRs, Virology, 375, 223, 10.1016/j.virol.2008.01.014 Alvarez, 2005, Long-range RNA-RNA interactions circularize the dengue virus genome, J. Virol., 79, 6631, 10.1128/JVI.79.11.6631-6643.2005 Benarroch, 2004, A structural basis for the inhibition of the NS5 dengue virus mRNA 2’-O-methyltransferase domain by ribavirin 5’-triphosphate, J. Biol. Chem., 279, 35638, 10.1074/jbc.M400460200 Crance, 2003, Interferon, ribavirin, 6-azauridine and glycyrrhizin: antiviral compounds active against pathogenic F flaviviruses, Antiviral Res., 58, 73, 10.1016/S0166-3542(02)00185-7 de Felipe, 2006, E unum pluribus: multiple proteins from a self-processing polyprotein, Trends Biotechnol., 24, 68, 10.1016/j.tibtech.2005.12.006 Dong, 2008, Flavivirus methyltransferase: A novel antiviral target, Antiviral Res., 80, 1, 10.1016/j.antiviral.2008.05.003 Doronina, 2008, Site-specific release of nascent chains from ribosomes at a sense codon, Mol. Cell. Biol., 28, 4227, 10.1128/MCB.00421-08 Egloff, 2002, An RNA cap (nucleoside-2’-O-)-methyltransferase in the flavivirus RNA polymerase NS5: crystal structure and functional characterization, EMBO J., 21, 2757, 10.1093/emboj/21.11.2757 Ekkapongpisit, 2007, cDNA-AFLP analysis of differential gene expression in human hepatoma cells (HepG2) upon dengue virus infection, J. Med. Virol., 79, 552, 10.1002/jmv.20806 Falgout, 1989, Proper processing of dengue virus nonstructural glycoprotein NS1 requires the N-terminal hydrophobic signal sequence and the downstream nonstructural protein NS2a, J. Virol., 63, 1852, 10.1128/JVI.63.5.1852-1860.1989 Fayzulin, 2006, Evaluation of replicative capacity and genetic stability of West Nile virus replicons using highly efficient packaging cell lines, Virology, 351, 196, 10.1016/j.virol.2006.02.036 Filomatori, 2006, A 5’ RNA element promotes dengue virus RNA synthesis on a circular genome, Genes Dev., 20, 2238, 10.1101/gad.1444206 Goodell, 2006, Identification of compounds with anti-West Nile Virus activity, J. Med. Chem., 49, 2127, 10.1021/jm051229y Graci, 2006, Mechanisms of action of ribavirin against distinct viruses, Rev. Med. Virol., 16, 37, 10.1002/rmv.483 Gu, 2006, Discovery of small molecule inhibitors of West Nile virus using a high-throughput sub-genomic replicon screen, Antiviral Res., 70, 39, 10.1016/j.antiviral.2006.01.005 Gualano, 1998, Identification of a major determinant of mouse neurovirulence of dengue virus type 2 using stably cloned genomic-length cDNA, J. Gen. Virol., 79, 437, 10.1099/0022-1317-79-3-437 Harvey, 2004, Tetracycline-inducible packaging cell line for production of flavivirus replicon particles, J. Virol., 78, 531, 10.1128/JVI.78.1.531-538.2004 Hellen, 2001, Internal ribosome entry sites in eukaryotic mRNA molecules, Genes Dev., 15, 1593, 10.1101/gad.891101 Hennecke, 2001, Composition and arrangement of genes define the strength of IRES-driven translation in bicistronic mRNAs, Nucleic Acids Res., 29, 3327, 10.1093/nar/29.16.3327 Hershkovitz, 2008, Dengue virus replicon expressing the nonstructural proteins suffices to enhance membrane expression of HLA class I and inhibit lysis by human NK cells, J. Virol., 82, 7666, 10.1128/JVI.02274-07 Higa, 2008, Secretome of HepG2 cells infected with dengue virus: implications for pathogenesis, Biochim. Biophys. Acta, 1784, 1607, 10.1016/j.bbapap.2008.06.015 Huerre, 2001, Liver histopathology and biological correlates in five cases of fatal dengue fever in Vietnamese children, Virchows Arch., 438, 107, 10.1007/s004280000329 Ibrahimi, 2009, Highly efficient multicistronic lentiviral vectors with peptide 2A sequences, Hum. Gene Ther., 20, 845, 10.1089/hum.2008.188 Johnston, 2007, HTS identifies novel and specific uncompetitive inhibitors of the two-component NS2B-NS3 proteinase of West Nile virus, Assay Drug Dev. Technol., 5, 737, 10.1089/adt.2007.101 Jones, 2005, Dengue virus inhibits alpha interferon signaling by reducing STAT2 expression, J. Virol., 79, 5414, 10.1128/JVI.79.9.5414-5420.2005 Kapoor, 1995, Association between NS3 and NS5 proteins of dengue virus type 2 in the putative RNA replicase is linked to differential phosphorylation of NS5, J. Biol. Chem., 270, 19100, 10.1074/jbc.270.32.19100 Khromykh, 2001, Essential role of cyclization sequences in flavivirus RNA replication, J. Virol., 75, 6719, 10.1128/JVI.75.14.6719-6728.2001 Koff, 1982, Antiviral effects if ribavirin and 6-mercapto-9-tetrahydro-2-furylpurine against dengue viruses in vitro, Antiviral Res., 2, 69, 10.1016/0166-3542(82)90027-4 Lescar, 2008, Towards the design of antiviral inhibitors against flaviviruses: the case for the multifunctional NS3 protein from Dengue virus as a target, Antiviral Res., 80, 94, 10.1016/j.antiviral.2008.07.001 Leung, 2008, Role of nonstructural protein NS2A in flavivirus assembly, J. Virol., 82, 4731, 10.1128/JVI.00002-08 Liang, 2005, Replication of a novel subgenomic HCV genotype 1a replicon expressing a puromycin resistance gene in Huh-7 cells, Virology, 333, 41, 10.1016/j.virol.2004.12.031 Lim, 2008, A scintillation proximity assay for dengue virus NS5 2’-O-methyltransferase-kinetic and inhibition analyses, Antiviral Res., 80, 360, 10.1016/j.antiviral.2008.08.005 Lindenbach, 2007, Flaviviridae: the viruses and their replication, 1101 Liu, 2005, Inhibition of interferon signaling by the New York 99 strain and Kunjin subtype of West Nile virus involves blockage of STAT1 and STAT2 activation by nonstructural proteins, J. Virol., 79, 1934, 10.1128/JVI.79.3.1934-1942.2005 Lo, 2003, Functional analysis of mosquito-borne flavivirus conserved sequence elements within 3’ untranslated region of West Nile virus by use of a reporting replicon that differentiates between viral translation and RNA replication, J. Virol., 77, 10004, 10.1128/JVI.77.18.10004-10014.2003 Lo, 2003, Potential high-throughput assay for screening inhibitors of West Nile virus replication, J. Virol., 77, 12901, 10.1128/JVI.77.23.12901-12906.2003 Malet, 2008, The flavivirus polymerase as a target for drug discovery, Antiviral Res., 80, 23, 10.1016/j.antiviral.2008.06.007 Mizuguchi, 2000, IRES-dependent second gene expression is significantly lower than cap-dependent first gene expression in a bicistronic vector, Mol. Ther., 1, 376, 10.1006/mthe.2000.0050 Ng, 2007, Construction and characterization of a stable subgenomic dengue virus type 2 replicon system for antiviral compound and siRNA testing, Antiviral Res., 76, 222, 10.1016/j.antiviral.2007.06.007 Noueiry, 2007, Identification of novel small-molecule inhibitors of West Nile virus infection, J. Virol., 81, 11992, 10.1128/JVI.01358-07 Parker, 2005, Metabolism and antiviral activity of ribavirin, Virus Res., 107, 165, 10.1016/j.virusres.2004.11.006 Pattanakitsakul, 2007, Proteomic analysis of host responses in HepG2 cells during dengue virus infection, J. Proteome Res., 6, 4592, 10.1021/pr070366b Pryor, 2001, Replication of dengue virus type 2 in human monocyte-derived macrophages: comparisons of isolates and recombinant viruses with substitutions at amino acid 390 in the envelope glycoprotein, Am. J. Trop. Med. Hyg., 65, 427, 10.4269/ajtmh.2001.65.427 Puig-Basagoiti, 2005, High-throughput assays using a luciferase-expressing replicon, virus-like particles, and full-length virus for West Nile virus drug discovery, Antimicrob. Agents Chemother., 49, 4980, 10.1128/AAC.49.12.4980-4988.2005 Puig-Basagoiti, 2009, Identification and characterization of inhibitors of West Nile virus, Antiviral Res., 83, 71, 10.1016/j.antiviral.2009.03.005 Puig-Basagoiti, 2006, Triaryl pyrazoline compound inhibits flavivirus RNA replication, Antimicrob. Agents Chemother., 50, 1320, 10.1128/AAC.50.4.1320-1329.2006 Rice, 1985, Nucleotide sequence of yellow fever virus: implications for flavivirus gene expression and evolution, Science, 229, 726, 10.1126/science.4023707 Rodrigo, 2006, Differential enhancement of dengue virus immune complex infectivity mediated by signaling-competent and signaling-incompetent human Fcgamma RIA (CD64) or FcgammaRIIA (CD32), J. Virol., 80, 10128, 10.1128/JVI.00792-06 Rossi, 2007, Mutations in West Nile virus nonstructural proteins that facilitate replicon persistence in vitro attenuate virus replication in vitro and in vivo, Virology, 364, 184, 10.1016/j.virol.2007.02.009 Rossi, 2005, Adaptation of West Nile virus replicons to cells in culture and use of replicon-bearing cells to probe antiviral action, Virology, 331, 457, 10.1016/j.virol.2004.10.046 Selisko, 2006, Comparative mechanistic studies of de novo RNA synthesis by flavivirus RNA-dependent RNA polymerases, Virology, 351, 145, 10.1016/j.virol.2006.03.026 Seneviratne, 2006, Pathogenesis of liver involvement during dengue viral infections, Trans. R. Soc. Trop. Med. Hyg., 100, 608, 10.1016/j.trstmh.2005.10.007 Shi, 2002, Construction and characterization of subgenomic replicons of New York strain of West Nile virus, Virology, 296, 219, 10.1006/viro.2002.1453 Sidwell, 1972, Broad-spectrum antiviral activity of Virazole: 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide, Science, 177, 705, 10.1126/science.177.4050.705 Streeter, 1973, Mechanism of action of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole), a new broad-spectrum antiviral agent, Proc. Natl. Acad. Sci. U.S.A., 70, 1174, 10.1073/pnas.70.4.1174 Suzuki, 2007, Construction of an infectious cDNA clone for a Brazilian prototype strain of dengue virus type 1: characterization of a temperature-sensitive mutation in NS1, Virology, 362, 374, 10.1016/j.virol.2006.11.026 Takhampunya, 2006, Inhibition of dengue virus replication by mycophenolic acid and ribavirin, J. Gen. Virol., 87, 1947, 10.1099/vir.0.81655-0 Tilgner, 2005, The flavivirus-conserved penta-nucleotide in the 3’ stem-loop of the West Nile virus genome requires a specific sequence and structure for RNA synthesis, but not for viral translation, Virology, 331, 375, 10.1016/j.virol.2004.07.022 Tilgner, 2004, Structure and function of the 3’ terminal six nucleotides of the west nile virus genome in viral replication, J. Virol., 78, 8159, 10.1128/JVI.78.15.8159-8171.2004 Yap, 2007, Crystal structure of the dengue virus RNA-dependent RNA polymerase catalytic domain at 1.85-angstrom resolution, J. Virol., 81, 4753, 10.1128/JVI.02283-06 You, 1999, A novel in vitro replication system for Dengue virus. Initiation of RNA synthesis at the 3’-end of exogenous viral RNA templates requires 5’- and 3’-terminal complementary sequence motifs of the viral RNA, J. Biol. Chem., 274, 33714, 10.1074/jbc.274.47.33714 Zhang, 1999, A simple statistical parameter for use in evaluation and validation of high throughput screening assays, J. Biomol. Screen., 4, 67, 10.1177/108705719900400206