Clonal fidelity investigation of micropropagated hardened plants of jackfruit tree (Artocarpus heterophyllus L.) with RAPD markers
Tóm tắt
Artocarpus heterophyllus is an important tropical agroforestry species that bears multiple applications. However, the population of this species is reduced due to various anthropogenic activities. For this reason, in vitro approach is needed to propagate or conserve this species as in vivo propagation methods face various obstacles. In this respect, the present investigation was undertaken to produce genetically stable jackfruit trees through in vitro technology. In vivo grew shoot tips were harvested on Murashige and Skoog (MS) medium containing several plant growth regulators to achieve this. The 6-benzylaminopurine (BAP) at the concentration of 1.5 mg L-1, indole-3-butyric acid (IBA) at 0.5 mg L-1, and α-naphthaleneacetic acid (NAA) at 0.1 mg L-1 in combination on MS media yielded superior shoot response (94.44%), longest shoot length (4.02 cm), and the maximum number of shoots per explant (4.78). They were further multiplied by repeated subculturing on the same media composition and the third subculture resulted in a maximum number of shoots (5.92) with the largest shoot length (5.85 cm). Among the different media screened for rooting, the ¼ MS media yielded 94.44% rooting response, the longest root length (3.78 cm), and the maximum number of roots per shoot (8.44) with 0.1 mg L-1 NAA, 0.5 mg L-1 IBA and 0.1 mg L-1 BAP in combination. Primary hardening showed 88.89% of plant survival under greenhouse conditions after 4 weeks of incubation having a sterilized mixture of garden soil and vermiculite mixture (1:1, w/w). It increased to 90.60% after the secondary hardening process in a vermiculite-soil mixture (2:1; w/w). No polymorphism was detected on random amplification of polymorphic DNA (RAPD) profiling between the mother plant and hardened plants, indicating high genetic stability among the clones. This is the first report of the genetic fidelity study of in vitro grown regenerants of A. heterophyllus. This study established a micropropagation protocol for genetically uniform in vitro regeneration of this species to supply plant resources to various industries or conservation of elite germplasm.
Tài liệu tham khảo
Ahmed MR, Anis M, Alatar AA, Faisal M (2017) In vitro clonal propagation and evaluation of genetic fidelity using RAPD and ISSR marker in micropropagated plants of Cassia alata L.: a potential medicinal plant. Agr Syst 91:637–647 https://link.springer.com/article/10.1007/s10457-016-9992-1
Ali J, Bantte K, Feyissa T (2017) Protocol optimization for in vitro shoot multiplication of jackfruit (Artocarpus heterophyllus L.). Afr J Biotechnol 16:87–90. https://doi.org/10.5897/AJB2015.15191
Amany HM, Ali EAM, Boshra ES (2007) In vitro propagation of jackfruit (Artocarpus heterophyllus L.). J Appl Sci Res 3:218–226
Amin MA, Jaiswal VS (1993) In vitro response of apical bud explants from mature tress of jackfruit (Artocarpus heterophyllus). Plant Cell Tiss Org Cult 33:59–65 https://link.springer.com/article/10.1007/BF01997599
Ara KA, Sharifuzzaman SM, Hossain MM, Talukder ASMHM (2000) Micropropagation of year-round jack fruit (Artocarpus heterophyllus Lam.) through in vitro culture. Ann Bangladesh Agric 10:85–91
Arora K, Sharma M, Srivastava J, Ranade SA, Sharma AK (2011) In vitro cloning of Azadirachta indica from root explants. Biol Plant 55:164–168 https://bp.ueb.cas.cz/pdfs/bpl/2011/01/23.pdf
Ashrafuzzaman M, Kar S, Khanam D, Prodhan SH (2012) In vitro regeneration and multiplication of Jackfruit (Artocarpus heterophyllus L.). J Biol 2:59–65
Azam FMS, Rahmatullah M, Zaman AU (2009) Tissue culture of a year-round fruiting variety of Artocarpus heterophyllus L. in Bangladesh. Acta Hortic 806:269–276. https://doi.org/10.17660/ActaHortic.2009.806.33
Bapat VA, Mhatre M, Rao PS (1987) Propagation of Morus indica L. (mulberry) by encapsulated shoot buds. Plant Cell Rep 6:393–395 https://link.springer.com/article/10.1007/BF00269570
Bhatia R, Singh KP, Sharma TR, Jhang T (2011) Evaluation of the genetic fidelity of in vitro-propagated gerbera (Gerbera jamesonii Bolus) using DNA-based markers. Plant Cell Tiss Org Cult 104:131–135. https://doi.org/10.1007/s11240-010-9806-5
Bindiya K, Kanwar K (2003) Random amplified polymorphic DNA (RAPDs) markers for genetic analysis in micropropagated plants of Robinia pseudoacacia L. Euphytica 135:41–47
Burci LM, da Silva CV, Rondon JN, da Silva LM, de Andrade SF, Miguel OG, Dias JFG, Miguel MD (2019) Acute and subacute (28 days) toxicity, hemolytic and cytotoxic effect of Artocarpus heterophyllus seed extracts. Toxicol Rep 6:1304–1308. https://doi.org/10.1016/j.toxrep.2018.02.006
Cassels AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tiss Org Cult 64:145–157 https://link.springer.com/article/10.1023/A:1010692104861
Chalageri G, Babu UV (2012) In vitro plant regeneration via petiole callus of Viola patrinii and genetic fidelity assessment using RAPD markers. Turk J Bot 36:358–368. https://doi.org/10.3906/bot-1102-7
Chaturvedi R, Razdan MK, Bhojwani SS (2004) In vitro clonal propagation of an adult tree of neem (Azadirachta indica A. Juss.) by forced axillary branching. Plant Sci 166:501–506. https://doi.org/10.1016/j.plantsci.2003.10.021
Cuesta C, Ordás RJ, Rodríguez A, Fernández B (2010) PCR-based molecular markers for assessment of somaclonal variation in Pinus pinea clones micro-propagated in vitro. Biol Plant 54:435–442 https://bp.ueb.cas.cz/pdfs/bpl/2010/03/06.pdf
De Klerk GJ (1990) How to measure somaclonal variation. Acta Bot Neerl 39:129–144
Devi PSS, Kumar NS, Sabu KK (2021) Phytochemical profiling and antioxidant activities of different parts of Artocarpus heterophyllus Lam. (Moraceae): A review on current status of knowledge. Future. J Pharm Sci 7:30. https://doi.org/10.1186/s43094-021-00178-7
Falcao MDA, Clement CR (2001) Phenology and yield of breadfruit (Artocarpus altilis) and jackfruit (Artocarpus heterophyllus) in Central Amazonia. Acta Amazon 31:179–191. https://doi.org/10.1590/1809-43922001312191
Geng F, Moran R, Day M, Halteman W, Zhang D (2016) Increasing in vitro shoot elongation and proliferation of ‘G.30’ and ‘G.41’ Apple by chilling explants and plant growth regulators. HortScience 51:899–904. https://doi.org/10.21273/HORTSCI.51.7.899
Ghareeb ZF, Taha LS (2018) Micropropagation protocol for Antigonon leptopus an important ornamental and medicinal plant. J Genet Eng Biotechnol 16:669–675. https://doi.org/10.1016/j.jgeb.2018.03.008
Ghosh PD, Chattopadhyay SK, Adhikari S, Saha S, Mondal S, Kader A, Dey ST, Bandyopadhyay TK, Sengupta C (2013) A high throughput DNA extraction method from chemotypically heterogeneous plant species. Protoc Exch. https://doi.org/10.1038/protex.2013.018
Grattapaglia D, Chaparro J, Wilcox P, McCord S, Werner D, Amerson H, McKeand S, Bridgwater S, Whetten R, O’Malley D, Sederoff R (1992) Mapping in woody plants with RAPD markers: application to breeding in forestry and horticulture. In: Proceedings of the Symposium Application of RAPD Technology to Plant Breeding, Joint Plant Breeding Symposia Series, Crop Science Society of America/American Society for Horticultural Science/American Genetic Association, Minneapolis, 37-40 November 1992
Haq N (2000) Report on Evaluation of Fruit Trees in Homesteads of Bangladesh and their possible marketing opportunities. DFID-SHABJE Project. CARE Bangladesh, Dhaka
Harb EM, Alhady MRAA, Elsalam NAA (2015) In vitro rapid propagation of jackfruit (Artcarpus heterophyllus Lam.). Am-Eur J Agric Environ Sci 15:147–153
Heinze B, Schmidt J (1995) Monitoring genetic fidelity vs somaclonal variation in Norway spruce (Picea abies) somatic embryogenesis by RAPD analysis. Euphytica 85:341–345
Hossain AKMA, Haq N (2006) Jackfruit, Artocarpus heterophyllus, Field Manual for Extension Workers and Farmers, SCUC, Southampton University, UK. https://www.yumpu.com/en/document/read/27317183/jackfruit-extension-manualpdf-crops-for-the-future
Ibáñez A, Valero M, Morte A (2003) Influence of cytokinins and subculturing on proliferation capacity of single-axillary-bud microcuttings of Vitis vinifera L. cv. Napoleón. Anal Biol 25:81–90
Jagadeesh SL, Reddy BS, Basavaraj N, Swamy GSK, Gorbal K, Hegde L, Raghavan GSV, Kajjidoni ST (2007) Inter tree variability for fruit quality in jackfruit selections of Western Ghats of India. Sci Hortic 112:382–387
Jagtap UB, Bapat VA (2010) Artocarpus: a review of traditional uses, ethnopharmacology and phytochemistry. J Ethnopharmacol 129:142–166
Jagtap UB, Bapat VA (2013) Green synthesis of silver nanoparticles using Artocarpus heterophyllus Lam. seed extract and its antibacterial activity. Ind Crop Prod 46:132–137
Jin S, Mushke R, Huaguo Z, Tu L, Lin Z, Zhang Y, Zhang X (2008) Detection of somaclonal variation of cotton (Gossypium hirsutum) using cytogenetics, flow cytometry and molecular markers. Plant Cell Rep 27:1303–1316 https://link.springer.com/article/10.1007/s00299-008-0557-2
Kamal NHM, Aziz MA, Kadzimin S, Rashid AA (2018) In Vitro mass multiplication of Artocarpus heterophyllus Lam var. tekam yellow. Pertanika J Trop Agric Sci 41:1289–1314
Kawiak A, Łojkowska E (2004) Application of RAPD in the determination of genetic fidelity in micropropagated Drosera plantlets. In Vitro Cell Dev Biol-Plant 40:592–595. https://doi.org/10.1079/IVP2004577
Khan R, Zerega N, Hossain S, Zuberi MI (2010) Jackfruit (Artocarpus heterophyllus Lam.) diversity in Bangladesh: land use and artificial selection. Econ Bot 64:124–136
Khan S, Saeed B, Kauser N (2011) Establishment of genetic fidelity of in-vitro raised banana plantlets. Pak J Bot 43:233–242
Kunhamu TK (2011) Jack and Agroforestry. In: Valavi SG, Peter KV, Thottappilly G (eds) The jackfruit. Studium Press LLC, Houston, pp 177–189
Lee CL, Keng CL (2005) Micropropagation of Artcarpus heterophyllus Lamk. Planter 81:689–695
Leroy XJ, Leon K, Charles G, Branchard M (2000) Cauliflower somatic embryogenesis and analysis of regenerant stability by ISSRs. Plant Cell Rep 19:1102–1107
Marum L, Rocheta M, Maroco J, Oliveira MM, Miguel C (2009) Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster). Plant Cell Rep 28:673–682. https://doi.org/10.1007/s00299-008-0668-9
McCown BH (2000) Recalcitrance of woody and herbaceous perennial plants: dealing with genetic predeterminism. In Vitro Cell Dev Biol Plant 36:149–154. https://doi.org/10.1007/s11627-000-0030-6
Mitra SK, Maity CS (2000) Genetic resources of Jackfruit (Artocarpus heterophyllus) in West Bengal, India. Acta Hortic 575:269–271
Munthali MT, Newbury HJ, Ford-Lloyd BV (1996) The detection of somaclonal variants of beet using RAPD. Plant Cell Rep 15:474–478
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Nissen SJ, Sutter EG (1990) Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience 25:800–802. https://doi.org/10.21273/HORTSCI.25.7.800
Orwa C, Mutua A, Kindt R, Jamnadass R, Anthony S (2009) Agroforestry database: a tree reference and selection guide version 4.0. World Agroforestry Centre, Kenya
Patel AK, Phulwaria M, Rai MK, Gupta AK, Shekhawat S, Shekhawat NS (2014) In vitro propagation and ex vitro rooting of Caralluma edulis (Edgew.) Benth. & Hook. f.: an endemic and endangered edible plant species of the Thar Desert. Sci Hortic 165:175–180. https://doi.org/10.1016/j.scienta.2013.10.039
Peschke VM, Phillips RL (1992) Genetic implications of somaclonal variation in plants. Adv Genet 30:41–75
Phillips RL, Kaeppler SM, Olhoft P (1994) Genetic stability of plant tissue cultures: breakdown of normal controls. Proc Natl Acad Sci U S A 91:5222–5226
Prakash O, Kumar R, Mishra A, Gupta R (2009) Artocarpus heterophyllus (Jackfruit): an overview. Pharmacogn Rev 3:353–358
Rafalski A, Tmgey S, Williams JGK (1993) Random amplified polymorphic DNA (RAPD) markers. In: Gelvin SB, Schilperoort RA, Verma DPS (eds) Plant Molecular Biology Manual. Kluwer, Dordrecht, pp 1–9
Rodrigues PHV, Tulmann Neto A, Cassieri Neto P, Mendes BMJ (1998) Influence of the number of subcultures on somaclonal variation in micropropagated Nanico (Musa spp., AAA group). Acta Hort 490:469–473
Rohlf FJ (2000) NTSYS-pc: numerical taxonomy and multivariate analysis system, version 2.1. Exeter Software, Setauket
Ryynanen L, Aronen T (2005) Genome fidelity during short- and long-term tissue culture and differentially cryostored meristems of silver birch (Betula pendula). Plant Cell Tiss Org Cult 83:21–32
Saha S, Kader A, Sengupta C, Ghosh P (2012) In vitro propagation of Ocimum gratissimum L. (Lamiaceae) and its evaluation of genetic fidelity using RAPD marker. Am J Plant Sci 3:64–74. https://doi.org/10.4236/ajps.2012.31006
Salvi ND, George L, Eapen S (2001) Plant regeneration from leaf base callus of turmeric and random amplified polymorphic DNA analysis of regenerated plants. Plant Cell Tiss Org Cult 66:113–119
Santos JRP, Teixeira MA, Cares JE, Faleiro FG, Costa DC (2010) Contrastant banana accessions for resistance to the burrowing nematode, based on molecular markers RAPD. Euphytica 172:13–20 https://link.springer.com/article/10.1007/s10681-009-0001-x
Sharma V, Belwal N, Kamal B, Dobriyal AK, Jadon VS (2016) Assessment of genetic fidelity of in vitro raised plants in Swertia chirayita through ISSR, RAPD analysis and peroxidase profiling during organogenesis. Braz Arch Biol Technol 59:e16160389. https://doi.org/10.1590/1678-4324-2016160389
Shekhawat MS, Kannan N, Manokari M, Ravindran CP (2015) Enhanced micropropagation protocol of Morinda citrifolia L. through nodal explants. J Appl Res Med Arom Plant 2:174–181. https://doi.org/10.1016/j.jarmap.2015.06.002
Shenoy VB, Vasil IK (1992) Biochemical and molecular analysis of plants derived from embryogenic tissue cultures of napier grass (Pennisetum purpureum K. Schum). Theor Appl Genet 83:947–955 https://link.springer.com/article/10.1007/BF00232955
Silvarolla MB, Mazzafera P, de Lima MMA (2000) Caffeine content of Ethiopian Coffea Arabica beans. Genet Mol Biol 23:213–215
Singh SR, Dalal S, Singh R, Dhawan AK, Kalia RK (2013) Evaluation of genetic fidelity of in vitro raised plants of Dendrocalamus asper (Schult. & Schult. F.) Backer ex K. Heyne using DNA-based markers. Acta Physiol Plant 35:419–430. https://doi.org/10.1007/s11738-012-1084-x
Siwach P, Gill AR (2014) Micropropagation of Ficus religiosa L. via leaf explants and comparative evaluation of acetylcholinesterase inhibitory activity in the micropropagated and conventionally grown plants. 3 Biotech 4:477–491 https://link.springer.com/article/10.1007/s13205-013-0175-8
Smith MK (1998) A review of factors influencing the genetic stability of micropropagated bananas. Fruits 43:219–223
Smulders MJM, Bredemeijer G, Rus-Kortekaas W, Arens P, Vosman B (1997) Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species. Theor Appl Genet 94:264–272 https://link.springer.com/article/10.1007/s001220050409
Sreedhar RV, Venkatachalam L, Bhagyalakshmi N (2007) Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers. Biotechnol J 2:1007–1013
Srivastava V, Khan SA, Banerjee S (2009) An evaluation of genetic fidelity of encapsulated microshoots of the medicinal plant: Cineraria maritima following six months of storage. Plant Cell Tiss Org Cult 99:193–198. https://doi.org/10.1007/s11240-009-9593-z
Stanišić M, Raspor M, Ninković S, Milošević S, Ćalić D, Bohanec B, Trifunović M, Petrić M, Subotić A, Jevremović S (2015) Clonal fidelity of Iris sibirica plants regenerated by somatic embryogenesis and organogenesis in leaf-base culture - RAPD and flow cytometer analyses. S Afr J Bot 96:42–52. https://doi.org/10.1016/j.sajb.2014.10.014
Torregrosa L, Bouquet A (1995) In vitro propagation of Vitis x Muscadinia hybrids by microcuttings or axillary budding. Vitis 34:237–238
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535. https://doi.org/10.1093/nar/18.22.6531
Yadav R, Yadav N, Kumar S (2015) An improved micropropagation and assessment of genetic fidelity in multipurpose medicinal tree, Acacia auriculiformis. Proc Natl Acad Sci India Sect B Biol Sci 86:921–929 https://link.springer.com/article/10.1007/s40011-015-0550-9
Yu J, Holland JB, McMullen MD, Buckler ES (2008) Genetic design and statistical power of nested association mapping in maize. Genetics 178:539–551. https://doi.org/10.1534/genetics.107.074245
Zhang H, Horgan KJ, Reynolds PH, Jameson PE (2010) 6-Benzyladenine metabolism during reinvigoration of mature Pinus radiate buds in vitro. Tree Physiol 30:514–526. https://doi.org/10.1093/treephys/tpp130