Transcriptome profiling of short-term response to chilling stress in tolerant and sensitive Oryza sativa ssp. Japonica seedlings

Springer Science and Business Media LLC - Tập 18 - Trang 627-644 - 2018
Matteo Buti1, Marianna Pasquariello2, Domenico Ronga1, Justyna Anna Milc1,3, Nicola Pecchioni3,4, Viet The Ho5,6, Chiara Pucciariello5, Pierdomenico Perata5, Enrico Francia1,3
1Biogest-Siteia, University of Modena and Reggio Emilia, Reggio Emilia, Italy
2Department of Crop Genetics, John Innes Centre, Norwich, UK
3Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy
4Cereal Research Centre, Council for Agricultural Research and Economics, Foggia, Italy
5PlantLab, Scuola Superiore Sant’Anna, Pisa, Italy
6Ho Chi Minh City University of Food Industry, Ho Chi Minh, Vietnam

Tóm tắt

Low temperature is a major factor limiting rice growth and yield, and seedling is one of the developmental stages at which sensitivity to chilling stress is higher. Tolerance to chilling is a complex quantitative trait, so one of the most effective approaches to identify genes and pathways involved is to compare the stress-induced expression changes between tolerant and sensitive genotypes. Phenotypic responses to chilling of 13 Japonica cultivars were evaluated, and Thaibonnet and Volano were selected as sensitive and tolerant genotypes, respectively. To thoroughly profile the short-term response of the two cultivars to chilling, RNA-Seq was performed on Thaibonnet and Volano seedlings after 0 (not stressed), 2, and 10 h at 10 °C. Differential expression analysis revealed that the ICE-DREB1/CBF pathway plays a primary role in chilling tolerance, mainly due to some important transcription factors involved (some of which had never been reported before). Moreover, the expression trends of some genes that were radically different between Thaibonnet and Volano (i.e., calcium-dependent protein kinases OsCDPK21 and OsCDPK23, cytochrome P450 monooxygenase CYP76M8, etc.) suggest their involvement in low temperature tolerance too. Density of differentially expressed genes along rice genome was determined and linked to the position of known QTLs: remarkable co-locations were reported, delivering an overview of genomic regions determinant for low temperature response at seedling stage. Our study contributes to a better understanding of the molecular mechanisms underlying rice response to chilling and provides a solid background for development of low temperature-tolerant germplasm.

Tài liệu tham khảo

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